PGR(Phospho S190) & PGR Protein Phosphorylation Antibody Pair
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Specification
Product Description
This protein phosphorylation antibody pair set comes with two antibodies, one against the PGR protein, and the other against the specific S190 phosphorylated site of PGR for use in in situ Proximity Ligation Assay. See Publication Reference below.
Reactivity
Human
Quality Control Testing
Dual recognition immunofluorescence result.
Representative image of Proximity Ligation Assay of protein phosphorylation. HeLa cells were stained with dual recognition antibody pair set, rabbit polyclonal antibody 1:1200 and mouse monoclonal antibody 1:50. Each red dot represents one single phosphorylated protein. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Supplied Product
Antibody pair set content:
1. Phospho-PGR S190 rabbit polyclonal antibody (20 ul)
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (0.02% sodium azide, 50% glycerol)
2. PGR mouse monoclonal antibody (40 ug)
In 1x PBS, pH 7.2
*Reagents are sufficient for at least 30-50 assays using recommended protocols.Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
In situ Proximity Ligation Assay (Cell)
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Gene Info — PGR
Entrez GeneID
5241Gene Name
PGR
Gene Alias
NR3C3, PR
Gene Description
progesterone receptor
Omim ID
607311Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce two isoforms, A and B. The two isoforms are identical except for the additional 165 amino acids found in the N-terminus of isoform A only, and mediate their own response genes and physiologic effects with little overlap. The location of transcription initiation for isoform B has not been clearly determined. [provided by RefSeq
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