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CARM1(phospho S228) & CARM1 Protein Phosphorylation Antibody Pair

  • Catalog # : DP0244
  • Visit Frequency :
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  • Specification
  • Reactivity:
  • Human
  • Quality Control Testing:
  • Dual recognition immunofluorescence result.

    QC Testing of DP0244
    Representative image of Proximity Ligation Assay of protein phosphorylation. HeLa cells were stained with dual recognition antibody pair set, rabbit polyclonal antibody 1:1200 and mouse monoclonal antibody 1:50. Each red dot represents one single phosphorylated protein. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
  • Supplied Product:
  • Antibody pair set content:
    1. Phospho-CARM1 S228 rabbit polyclonal antibody (20 ul)
    In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (0.02% sodium azide, 50% glycerol)
    2. CARM1 mouse monoclonal antibody (40 ug)
    In 1x PBS, pH 7.2
    *Reagents are sufficient for at least 30-50 assays using recommended protocols.
  • Storage Instruction:
  • Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
  • Publication Reference
  • Applications
  • In situ Proximity Ligation Assay (Cell)
  • Application Image
  • In situ Proximity Ligation Assay (Cell)
  • Gene Information
  • Gene Name:
  • CARM1
  • Gene Alias:
  • PRMT4
  • Gene Description:
  • coactivator-associated arginine methyltransferase 1
  • Gene Summary:
  • Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in signal transduction, metabolism of nascent pre-RNA, and transcriptional activation (Frankel et al., 2002 [PubMed 11724789]).[supplied by OMIM
  • Other Designations:
  • coactivator-associated arginine methyltransferase-1,protein arginine N-methyltransferase 4
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