RPS6KB1(phospho S411) & RPS6KB1 Protein Phosphorylation Antibody Pair

Catalog # DP0221

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Size:1 Set
Price: USD $ 607.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
QC Test

Representative image of Proximity Ligation Assay of protein phosphorylation. HeLa cells were stained with dual recognition antibody pair set, rabbit polyclonal antibody 1:1200 and mouse monoclonal antibody 1:50. Each red dot represents one single phosphorylated protein. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.

  • Specification

    Product Description

    This protein phosphorylation antibody pair set comes with two antibodies, one against the RPS6KB1 protein, and the other against the specific S411 phosphorylated site of RPS6KB1 for use in in situ Proximity Ligation Assay. See Publication Reference below.

    Reactivity

    Human

    Quality Control Testing

    Dual recognition immunofluorescence result.

    Representative image of Proximity Ligation Assay of protein phosphorylation. HeLa cells were stained with dual recognition antibody pair set, rabbit polyclonal antibody 1:1200 and mouse monoclonal antibody 1:50. Each red dot represents one single phosphorylated protein. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.

    Supplied Product

    Antibody pair set content:
    1. Phospho-RPS6KB1 S411 rabbit polyclonal antibody (20 ul)
    In PBS, 150 mM NaCl, pH 7.4 (0.02% sodium azide, 50% glycerol)
    2. RPS6KB1 mouse monoclonal antibody (40 ug)
    In 1x PBS, pH 7.2
    *Reagents are sufficient for at least 30-50 assays using recommended protocols.

    Storage Instruction

    Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.

  • Applications

    In situ Proximity Ligation Assay (Cell)

  • Gene Info — RPS6KB1

    Entrez GeneID

    6198

    Gene Name

    RPS6KB1

    Gene Alias

    PS6K, S6K, S6K1, STK14A, p70(S6K)-alpha, p70-S6K, p70-alpha

    Gene Description

    ribosomal protein S6 kinase, 70kDa, polypeptide 1

    Omim ID

    608938

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized. [provided by RefSeq

    Other Designations

    p70 S6 kinase, alpha 1|p70 S6 kinase, alpha 2|ribosomal protein S6 kinase, 70kD, polypeptide 1|serine/threonine kinase 14 alpha

  • Interactome
  • Pathway
  • Disease
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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