MAP2K2(phospho T394) & MAP2K2 Protein Phosphorylation Antibody Pair
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Specification
Product Description
This protein phosphorylation antibody pair set comes with two antibodies, one against the MAP2K2 protein, and the other against the specific T394 phosphorylated site of MAP2K2 for use in in situ Proximity Ligation Assay. See Publication Reference below.
Reactivity
Human
Quality Control Testing
Dual recognition immunofluorescence result.
Representative image of Proximity Ligation Assay of protein phosphorylation. HeLa cells were stained with dual recognition antibody pair set, rabbit polyclonal antibody 1:1200 and mouse monoclonal antibody 1:50. Each red dot represents one single phosphorylated protein. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Supplied Product
Antibody pair set content:
1. Phospho-MAP2K2 T394 rabbit polyclonal antibody (20 ul)
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (0.02% sodium azide, 50% glycerol)
2. MAP2K2 mouse monoclonal antibody (40 ug)
In 1x PBS, pH 7.2
*Reagents are sufficient for at least 30-50 assays using recommended protocols.Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
In situ Proximity Ligation Assay (Cell)
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Gene Info — MAP2K2
Entrez GeneID
5605Gene Name
MAP2K2
Gene Alias
FLJ26075, MAPKK2, MEK2, MKK2, PRKMK2
Gene Description
mitogen-activated protein kinase kinase 2
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq
Other Designations
ERK activator kinase 2|MAP kinase kinase 2|MAPK/ERK kinase 2|dual specificity mitogen-activated protein kinase kinase 2|mitogen-activated protein kinase kinase 2, p45
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