TWF1(phospho Y321) & TWF1 Protein Phosphorylation Antibody Pair
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Specification
Product Description
This protein phosphorylation antibody pair set comes with two antibodies, one against the TWF1 protein, and the other against the specific Y321 phosphorylated site of TWF1 for use in in situ Proximity Ligation Assay. See Publication Reference below.
Reactivity
Human
Quality Control Testing
Dual recognition immunofluorescence result.
Representative image of Proximity Ligation Assay of protein phosphorylation. HeLa cells were stained with dual recognition antibody pair set, rabbit polyclonal antibody 1:1200 and mouse purified polyclonal antibody 1:50. Each red dot represents one single phosphorylated protein. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Supplied Product
Antibody pair set content:
1. Phospho-TWF1 Y321 rabbit polyclonal antibody (20 ul)
In PBS (0.09% (w/v) sodium azide)
2. TWF1 mouse purified polyclonal antibody (40 ug)
In 1x PBS, pH 7.2
*Reagents are sufficient for at least 30-50 assays using recommended protocols.Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
In situ Proximity Ligation Assay (Cell)
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Gene Info — TWF1
Entrez GeneID
5756Gene Name
TWF1
Gene Alias
A6, MGC23788, MGC41876, PTK9
Gene Description
twinfilin, actin-binding protein, homolog 1 (Drosophila)
Omim ID
610932Gene Ontology
HyperlinkGene Summary
This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1. [provided by RefSeq
Other Designations
A6 protein tyrosine kinase|PTK9 protein tyrosine kinase 9|protein tyrosine kinase 9|twinfilin 1
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