PAK1(phospho T212) & PAK1 Protein Phosphorylation Antibody Pair

Catalog # DP0058

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Size:1 Set
Price: USD $ 607.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
QC Test

Representative image of Proximity Ligation Assay of protein phosphorylation. HeLa cells were stained with dual recognition antibody pair set, rabbit polyclonal antibody 1:1200 and mouse monoclonal antibody 1:50. Each red dot represents one single phosphorylated protein. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.

  • Specification

    Product Description

    This protein phosphorylation antibody pair set comes with two antibodies, one against the PAK1 protein, and the other against the specific T212 phosphorylated site of PAK1 for use in in situ Proximity Ligation Assay. See Publication Reference below.

    Reactivity

    Human

    Quality Control Testing

    Dual recognition immunofluorescence result.

    Representative image of Proximity Ligation Assay of protein phosphorylation. HeLa cells were stained with dual recognition antibody pair set, rabbit polyclonal antibody 1:1200 and mouse monoclonal antibody 1:50. Each red dot represents one single phosphorylated protein. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.

    Supplied Product

    Antibody pair set content:
    1. Phospho-PAK1 T212 rabbit polyclonal antibody (20 ul)
    With 0.09% sodium azide.
    2. PAK1 mouse monoclonal antibody (40 ug)
    In 1x PBS, pH 7.2
    *Reagents are sufficient for at least 30-50 assays using recommended protocols.

    Storage Instruction

    Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.

  • Applications

    In situ Proximity Ligation Assay (Cell)

  • Gene Info — PAK1

    Entrez GeneID

    5058

    Gene Name

    PAK1

    Gene Alias

    MGC130000, MGC130001, PAKalpha

    Gene Description

    p21 protein (Cdc42/Rac)-activated kinase 1

    Omim ID

    602590

    Gene Ontology

    Hyperlink

    Gene Summary

    PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternativelt spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq

    Other Designations

    STE20 homolog, yeast|p21-activated kinase 1|p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)|p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)

  • Interactome
  • Pathway
  • Disease
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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