IKBKG(phospho S31) & IKBKG Protein Phosphorylation Antibody Pair
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Specification
Product Description
This protein phosphorylation antibody pair set comes with two antibodies, one against the IKBKG protein, and the other against the specific S31 phosphorylated site of IKBKG for use in in situ Proximity Ligation Assay. See Publication Reference below.
Reactivity
Human
Quality Control Testing
Dual recognition immunofluorescence result.
Representative image of Proximity Ligation Assay of protein phosphorylation. HeLa cells were stained with dual recognition antibody pair set, rabbit polyclonal antibody 1:1200 and mouse monoclonal antibody 1:50. Each red dot represents one single phosphorylated protein. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Supplied Product
Antibody pair set content:
1. Phospho-IKBKG S31 rabbit polyclonal antibody (20 ul)
With 0.09% sodium azide.
2. IKBKG mouse monoclonal antibody (40 ug)
In 1x PBS, pH 7.2
*Reagents are sufficient for at least 30-50 assays using recommended protocols.Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
In situ Proximity Ligation Assay (Cell)
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Gene Info — IKBKG
Entrez GeneID
8517Gene Name
IKBKG
Gene Alias
AMCBX1, FIP-3, FIP3, Fip3p, IKK-gamma, IP, IP1, IP2, IPD2, NEMO
Gene Description
inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
Gene Ontology
HyperlinkGene Summary
This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome. [supplied by RefSeq
Other Designations
NFkappaB essential modulator|OTTHUMP00000026027|OTTHUMP00000026028|OTTHUMP00000026029|incontinentia pigmenti
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Disease
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