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- Home > Products > Ab Pairs/Kits > Ab Pairs for PP > DNMT1(phospho Y399) & DNMT1 Protein Phosphorylation Antibody Pair
DNMT1(phospho Y399) & DNMT1 Protein Phosphorylation Antibody Pair
- Catalog # : DP0002
- Visit Frequency :
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- 1 Set
- USD $ 589
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- Specification
- Product Description:
- This protein phosphorylation antibody pair set comes with two antibodies, one against the DNMT1 protein, and the other against the specific Y399 phosphorylated site of DNMT1 for use in in situ Proximity Ligation Assay. See Publication Reference below.
- Reactivity:
- Human
- Quality Control Testing:
- Dual recognition immunofluorescence result.
Representative image of Proximity Ligation Assay of protein phosphorylation. HeLa cells were stained with dual recognition antibody pair set, rabbit polyclonal antibody 1:1200 and mouse monoclonal antibody 1:50. Each red dot represents one single phosphorylated protein. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
- Supplied Product:
- Antibody pair set content:
1. Phospho-DNMT1 Y399 rabbit polyclonal antibody (20 ul)
In PBS (0.09% (w/v) sodium azide)
2. DNMT1 mouse monoclonal antibody (40 ug)
In 1x PBS, pH 7.2
*Reagents are sufficient for at least 30-50 assays using recommended protocols.
- Storage Instruction:
- Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
- 1.
- In situ detection of phosphorylated platelet-derived growth factor receptor beta using a generalized proximity ligation method.
Jarvius M, Paulsson J, Weibrecht I, Leuchowius KJ, Andersson AC, Wahlby C, Gullberg M,Botling J, Sjoblom T, Markova B, Ostman A, Landegren U, Soderberg O.
Mol Cell Proteomics. 2007 Sep;6(9):1500-9. Epub 2007 Jun 12.
- 2.
- Direct observation of individual endogenous protein complexes in situ by proximity ligation.
Soderberg O, Gullberg M, Jarvius M , Ridderstrale K, Leuchowius KJ, Jarvius J, Wester K, Hydbring P, Bahram F, Larsson LG, and Landegren U.
Nat Methods. 2006 Dec;3(12):995-1000. Epub 2006 Oct 29.
- 3.
- Cytokine detection by antibody-based proximity ligation.
Gullberg M, Gustafsdottir SM, Schallmeiner E, Jarvius J, Bjarnegard M, Betsholtz C, Landegren U, and Fredriksson S.
Proc Natl Acad Sci U S A. 2004 Jun 1;101(22):8420-4. Epub 2004 May 21.
- 4.
- Protein detection using proximity-dependent DNA ligation assays.
Fredriksson S, GullbergM, Jarvius J, Olsson C, Pietras K, Gustafsdottir SM, Ostman A, and Landegren U.
Nat Biotechnol. 2002 May;20(5):473-7.
- 5.
- Highly specific detection of phosphorylated proteins by Duolink
Mats Gullberg and Ann-Catrin Andersson
Nature Methods 6. 2009
- Application Image
- In situ Proximity Ligation Assay (Cell)
- Entrez GeneID:
- 1786
- Gene Name:
- DNMT1
- Gene Alias:
- AIM,CXXC9,DNMT,FLJ16293,MCMT,MGC104992
- Gene Description:
- DNA (cytosine-5-)-methyltransferase 1
- Omim ID:
- 126375
- Gene Ontology:
- Hyperlink
- Gene Summary:
- DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
- Other Designations:
- CXXC finger protein 9,DNA methyltransferase 1
- Gene Pathway
- Related Disease
