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SUFU & GLI1 Protein Protein Interaction Antibody Pair

  • Catalog # : DI0625
  • Visit Frequency :
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  • Specification
  • Reactivity:
  • Human
  • Quality Control Testing:
  • Protein protein interaction immunofluorescence result.

    QC Testing of DI0625
    Representative image of Proximity Ligation Assay of protein-protein interactions between SUFU and GLI1. HeLa cells were stained with anti-SUFU rabbit purified polyclonal antibody 1:1200 and anti-GLI1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
  • Supplied Product:
  • Antibody pair set content:
    1. SUFU rabbit purified polyclonal antibody (20 ug)
    2. GLI1 mouse monoclonal antibody (40 ug)
    *Reagents are sufficient for at least 30-50 assays using recommended protocols.
  • Storage Instruction:
  • Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
  • Publication Reference
  • Applications
  • In situ Proximity Ligation Assay (Cell)
  • Application Image
  • In situ Proximity Ligation Assay (Cell)
  • Gene Information
  • Gene Name:
  • SUFU
  • Gene Alias:
  • PRO1280,SUFUH,SUFUXL
  • Gene Description:
  • suppressor of fused homolog (Drosophila)
  • Gene Summary:
  • SUFU encodes a component of the Sonic hedgehog (SHH; MIM 600725)/Patched (PTCH; MIM 601309) signaling pathway. Mutations in genes encoding components of this pathway are deleterious for normal development and are associated with cancer-predisposing syndromes (e.g., HPE3, MIM 142945; BCNS, MIM 109400; and GCPS, MIM 175700).[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000020374,OTTHUMP00000059161,suppressor of fused
  • Gene Information
  • Entrez GeneID:
  • 2735
  • Gene Name:
  • GLI1
  • Gene Alias:
  • GLI
  • Gene Description:
  • glioma-associated oncogene homolog 1 (zinc finger protein)
  • Gene Summary:
  • This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • glioma-associated oncogene homolog (zinc finger protein),glioma-associated oncogene homolog 1
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