This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the RPS6KA3 protein, and the other against the PDPK1 protein for use in in situ Proximity Ligation Assay. See Publication Reference below.
Reactivity:
Human
Quality Control Testing:
Protein protein interaction immunofluorescence result.
Representative image of Proximity Ligation Assay of protein-protein interactions between RPS6KA3 and PDPK1. HeLa cells were stained with anti-RPS6KA3 rabbit purified polyclonal antibody 1:1200 and anti-PDPK1 mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Supplied Product:
Antibody pair set content: 1. RPS6KA3 rabbit purified polyclonal antibody (100 ug) 2. PDPK1 mouse purified polyclonal antibody (40 ug) *Reagents are sufficient for at least 30-50 assays using recommended protocols.
Storage Instruction:
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
Representative image of Proximity Ligation Assay of protein-protein interactions between RPS6KA3 and PDPK1. A-549 cells were stained with anti-RPS6KA3 rabbit purified polyclonal antibody 1:100 and anti-PDPK1 mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between RPS6KA3 and PDPK1. HT-29 cells were stained with anti-RPS6KA3 rabbit purified polyclonal antibody 1:100 and anti-PDPK1 mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
In situ Proximity Ligation Assay (Cell)
Confocal microscopy image of Proximity Ligation Assay of protein-protein interactions between RPS6KA3 and PDPK1. HT-29 cells were stained with anti-RPS6KA3 rabbit purified polyclonal antibody 1:100 and anti-PDPK1 mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq
Other Designations:
OTTHUMP00000023036,insulin-stimulated protein kinase 1,mental retardation, X-linked 19,ribosomal protein S6 kinase, 90kD, polypeptide 3