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Last updated: 2017/7/23
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RPS6KA3 & PDPK1 Protein Protein Interaction Antibody Pair

  • Catalog # : DI0623
  • Visit Frequency :
  • Countries :
  • Specification
  • Reactivity:
  • Human
  • Quality Control Testing:
  • Protein protein interaction immunofluorescence result.

    QC Testing of DI0623
    Representative image of Proximity Ligation Assay of protein-protein interactions between RPS6KA3 and PDPK1. HeLa cells were stained with anti-RPS6KA3 rabbit purified polyclonal antibody 1:1200 and anti-PDPK1 mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
  • Supplied Product:
  • Antibody pair set content:
    1. RPS6KA3 rabbit purified polyclonal antibody (20 ug)
    2. PDPK1 mouse purified polyclonal antibody (40 ug)
    *Reagents are sufficient for at least 30-50 assays using recommended protocols.
  • Storage Instruction:
  • Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
  • Publication Reference
  • Applications
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • Representative image of Proximity Ligation Assay of protein-protein interactions between RPS6KA3 and PDPK1. A-549 cells were stained with anti-RPS6KA3 rabbit purified polyclonal antibody 1:100 and anti-PDPK1 mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • Representative image of Proximity Ligation Assay of protein-protein interactions between RPS6KA3 and PDPK1. HT-29 cells were stained with anti-RPS6KA3 rabbit purified polyclonal antibody 1:100 and anti-PDPK1 mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • Confocal microscopy image of Proximity Ligation Assay of protein-protein interactions between RPS6KA3 and PDPK1. HT-29 cells were stained with anti-RPS6KA3 rabbit purified polyclonal antibody 1:100 and anti-PDPK1 mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
  • Application Image
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • enlarge
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • enlarge
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 6197
  • Gene Name:
  • RPS6KA3
  • Gene Alias:
  • CLS,HU-3,ISPK-1,MAPKAPK1B,MRX19,RSK,RSK2,S6K-alpha3,p90-RSK2,pp90RSK2
  • Gene Description:
  • ribosomal protein S6 kinase, 90kDa, polypeptide 3
  • Gene Summary:
  • This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000023036,insulin-stimulated protein kinase 1,mental retardation, X-linked 19,ribosomal protein S6 kinase, 90kD, polypeptide 3
  • Gene Information
  • Entrez GeneID:
  • 5170
  • Gene Name:
  • PDPK1
  • Gene Alias:
  • MGC20087,MGC35290,PDK1,PRO0461
  • Gene Description:
  • 3-phosphoinositide dependent protein kinase-1
  • Other Designations:
  • PkB kinase like gene 1,PkB-like 1,protein kinase
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