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Last updated: 2017/12/10
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HDAC2 & HDAC1 Protein Protein Interaction Antibody Pair

  • Catalog # : DI0614
  • Visit Frequency :
  • Countries :
  • Specification
  • Reactivity:
  • Human
  • Quality Control Testing:
  • Protein protein interaction immunofluorescence result.

    QC Testing of DI0614
    Representative image of Proximity Ligation Assay of protein-protein interactions between HDAC2 and HDAC1. HeLa cells were stained with anti-HDAC2 rabbit purified polyclonal antibody 1:1200 and anti-HDAC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
  • Supplied Product:
  • Antibody pair set content:
    1. HDAC2 rabbit purified polyclonal antibody (20 ug)
    2. HDAC1 mouse monoclonal antibody (40 ug)
    *Reagents are sufficient for at least 30-50 assays using recommended protocols.
  • Storage Instruction:
  • Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
  • Publication Reference
  • Applications
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • Representative image of Proximity Ligation Assay of protein-protein interactions between HDAC2 and HDAC1. PC-3 cells were stained with anti-HDAC2 rabbit purified polyclonal antibody 1:100 and anti-HDAC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • Representative image of Proximity Ligation Assay of protein-protein interactions between HDAC2 and HDAC1. A-549 cells were stained with anti-HDAC2 rabbit purified polyclonal antibody 1:100 and anti-HDAC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • Representative image of Proximity Ligation Assay of protein-protein interactions between HDAC2 and HDAC1. HT-29 cells were stained with anti-HDAC2 rabbit purified polyclonal antibody 1:100 and anti-HDAC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • Confocal microscopy image of Proximity Ligation Assay of protein-protein interactions between HDAC2 and HDAC1. PC-3 cells were stained with anti-HDAC2 rabbit purified polyclonal antibody 1:100 and anti-HDAC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • Confocal microscopy image of Proximity Ligation Assay of protein-protein interactions between HDAC2 and HDAC1. A-549 cells were stained with anti-HDAC2 rabbit purified polyclonal antibody 1:100 and anti-HDAC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • Confocal microscopy image of Proximity Ligation Assay of protein-protein interactions between HDAC2 and HDAC1. HT-29 cells were stained with anti-HDAC2 rabbit purified polyclonal antibody 1:100 and anti-HDAC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
  • Application Image
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • enlarge
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • enlarge
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • enlarge
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • enlarge
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • enlarge
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 3066
  • Gene Name:
  • HDAC2
  • Gene Alias:
  • RPD3,YAF1
  • Gene Description:
  • histone deacetylase 2
  • Gene Summary:
  • This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones (H2A, H2B, H3 and H4). This protein also forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus it plays an important role in transcriptional regulation, cell cycle progression and developmental events. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000040427,YY1-associated factor 1,transcriptional regulator homolog RPD3
  • Gene Information
  • Entrez GeneID:
  • 3065
  • Gene Name:
  • HDAC1
  • Gene Alias:
  • DKFZp686H12203,GON-10,HD1,RPD3,RPD3L1
  • Gene Description:
  • histone deacetylase 1
  • Gene Summary:
  • Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000008745,reduced potassium dependency, yeast homolog-like 1
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