FASLG & FN1 Protein Protein Interaction Antibody Pair
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Specification
Product Description
This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the FASLG protein, and the other against the FN1 protein for use in in situ Proximity Ligation Assay. See Publication Reference below.
Reactivity
Human
Quality Control Testing
Protein protein interaction immunofluorescence result.
Representative image of Proximity Ligation Assay of protein-protein interactions between FASLG and FN1. HeLa cells were stained with anti-FASLG rabbit purified polyclonal antibody 1:1200 and anti-FN1 mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Supplied Product
Antibody pair set content:
1. FASLG rabbit purified polyclonal antibody (100 ug)
2. FN1 mouse purified polyclonal antibody (40 ug)
*Reagents are sufficient for at least 30-50 assays using recommended protocols.Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
In situ Proximity Ligation Assay (Cell)
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Gene Info — FASLG
Entrez GeneID
356Gene Name
FASLG
Gene Alias
APT1LG1, CD178, CD95L, FASL, TNFSF6
Gene Description
Fas ligand (TNF superfamily, member 6)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is the ligand for FAS. Both are transmembrane proteins. Interaction of FAS with this ligand is critical in triggering apoptosis of some types of cells such as lymphocytes. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). [provided by RefSeq
Other Designations
CD95 ligand|OTTHUMP00000032708|apoptosis (APO-1) antigen ligand 1|fas ligand|tumor necrosis factor (ligand) superfamily, member 6
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Gene Info — FN1
Entrez GeneID
2335Gene Name
FN1
Gene Alias
CIG, DKFZp686F10164, DKFZp686H0342, DKFZp686I1370, DKFZp686O13149, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF
Gene Description
fibronectin 1
Omim ID
135600Gene Ontology
HyperlinkGene Summary
This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq
Other Designations
cold-insoluble globulin|migration-stimulating factor
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Interactome
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Pathway
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Disease
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