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Last updated: 2017/8/20

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RHOA & DAAM1 Protein Protein Interaction Antibody Pair

  • Catalog # : DI0603
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  • Specification
  • Reactivity:
  • Human
  • Quality Control Testing:
  • Protein protein interaction immunofluorescence result.

    QC Testing of DI0603
    Representative image of Proximity Ligation Assay of protein-protein interactions between RHOA and DAAM1. HeLa cells were stained with anti-RHOA rabbit purified polyclonal antibody 1:1200 and anti-DAAM1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
  • Supplied Product:
  • Antibody pair set content:
    1. RHOA rabbit purified polyclonal antibody (20 ug)
    2. DAAM1 mouse monoclonal antibody (40 ug)
    *Reagents are sufficient for at least 30-50 assays using recommended protocols.
  • Storage Instruction:
  • Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
  • Publication Reference
  • Applications
  • In situ Proximity Ligation Assay (Cell)
  • Application Image
  • In situ Proximity Ligation Assay (Cell)
  • Gene Information
  • Entrez GeneID:
  • 387
  • Gene Name:
  • RHOA
  • Gene Alias:
  • ARH12,ARHA,RHO12,RHOH12
  • Gene Description:
  • ras homolog gene family, member A
  • Other Designations:
  • Aplysia ras-related homolog 12,oncogene RHO H12,small GTP binding protein RhoA
  • Gene Information
  • Gene Name:
  • DAAM1
  • Gene Alias:
  • FLJ41657,KIAA0666
  • Gene Description:
  • dishevelled associated activator of morphogenesis 1
  • Gene Summary:
  • Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton and recent evidence suggests a role for the Formin homology (FH) proteins in these processes. The protein encoded by this gene contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by this gene product, which is thought to function as a scaffolding protein. Evidence of alternative splicing has been observed for this gene but the full-length nature of these variants has not been determined. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000179033,dishevelled-associated activator of morphogenesis 1
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