STK36 & GLI1 Protein Protein Interaction Antibody Pair
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More Files
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Representative image of Proximity Ligation Assay of protein-protein interactions between STK36 and GLI1. HeLa cells were stained with anti-STK36 rabbit purified polyclonal antibody 1:1200 and anti-GLI1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
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Specification
Product Description
This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the STK36 protein, and the other against the GLI1 protein for use in in situ Proximity Ligation Assay. See Publication Reference below.
Reactivity
Human
Quality Control Testing
Protein protein interaction immunofluorescence result.
Representative image of Proximity Ligation Assay of protein-protein interactions between STK36 and GLI1. HeLa cells were stained with anti-STK36 rabbit purified polyclonal antibody 1:1200 and anti-GLI1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Supplied Product
Antibody pair set content:
1. STK36 rabbit purified polyclonal antibody (100 ug)
2. GLI1 mouse monoclonal antibody (40 ug)
*Reagents are sufficient for at least 30-50 assays using recommended protocols.Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
In situ Proximity Ligation Assay (Cell)
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Gene Info — GLI1
Entrez GeneID
2735Gene Name
GLI1
Gene Alias
GLI
Gene Description
glioma-associated oncogene homolog 1 (zinc finger protein)
Omim ID
165220Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
glioma-associated oncogene homolog (zinc finger protein)|glioma-associated oncogene homolog 1
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Gene Info — STK36
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Interactome
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Pathway
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Disease
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