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E2F2 & E2F1 Protein Protein Interaction Antibody Pair

Catalog # : DI0571
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Specification

Product Description:
This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the E2F2 protein, and the other against the E2F1 protein for use in in situ Proximity Ligation Assay. See Publication Reference below.

Reactivity:
Human

Quality Control Testing:
Protein protein interaction immunofluorescence result.

Representative image of Proximity Ligation Analysis of protein-protein interactions between E2F2 and E2F1. HeLa cells were stained with anti-E2F2 rabbit purified polyclonal antibody 1:100 and anti-E2F1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.

Supplied Product:
Antibody pair set content:
1. E2F2 rabbit purified polyclonal antibody (20 ug)
2. E2F1 mouse monoclonal antibody (40 ug)
*Reagents are sufficient for at least 30-50 assays using recommended protocols.

Storage Instruction:
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.

MSDS:
Download

Publication Reference

1.
An analysis of protein-protein interactions in cross-talk pathways reveals CRKL as a novel prognostic marker in hepatocellular carcinoma.
Liu CH, Chen TC, Chau GY, Jan YH, Chen CH, Hsu CN, Lin KT, Juang YL, Lu PJ, Cheng HC, Chen MH, Chang CF, Ting YS, Kao CY, Hsiao M, Huang CY. Mol Cell Proteomics. 2013 Feb 8. [Epub ahead of print]

Applications

In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between E2F2 and E2F1. PC-3 cells were stained with anti-E2F2 rabbit purified polyclonal antibody 1:100 and anti-E2F1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between E2F2 and E2F1. A-549 cells were stained with anti-E2F2 rabbit purified polyclonal antibody 1:100 and anti-E2F1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between E2F2 and E2F1. HT-29 cells were stained with anti-E2F2 rabbit purified polyclonal antibody 1:100 and anti-E2F1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

In situ Proximity Ligation Assay (Cell)
Confocal microscopy image of Proximity Ligation Assay of protein-protein interactions between E2F2 and E2F1. A-549 cells were stained with anti-E2F2 rabbit purified polyclonal antibody 1:100 and anti-E2F1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

In situ Proximity Ligation Assay (Cell)
Confocal microscopy image of Proximity Ligation Assay of protein-protein interactions between E2F2 and E2F1. HT-29 cells were stained with anti-E2F2 rabbit purified polyclonal antibody 1:100 and anti-E2F1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Application Image

In situ Proximity Ligation Assay (Cell)
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In situ Proximity Ligation Assay (Cell)
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In situ Proximity Ligation Assay (Cell)
enlarge

In situ Proximity Ligation Assay (Cell)
enlarge

In situ Proximity Ligation Assay (Cell)
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E2F1
E2F2

Gene Information

Entrez GeneID:
1870

Gene Name:
E2F2

Gene Alias:
E2F-2

Gene Description:
E2F transcription factor 2

Omim ID:
600426

Gene Ontology:
Hyperlink

Gene Summary:
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1. [provided by RefSeq

Other Designations:
OTTHUMP00000003257

Gene Information

Entrez GeneID:
1869

Gene Name:
E2F1

Gene Alias:
E2F-1,RBAP1,RBBP3,RBP3

Gene Description:
E2F transcription factor 1

Omim ID:
189971

Gene Ontology:
Hyperlink

Gene Summary:
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq