RPS6KA5 & EGF Protein Protein Interaction Antibody Pair
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Specification
Product Description
This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the RPS6KA5 protein, and the other against the EGF protein for use in in situ Proximity Ligation Assay. See Publication Reference below.
Reactivity
Human
Quality Control Testing
Protein protein interaction immunofluorescence result.
Representative image of Proximity Ligation Assay of protein-protein interactions between RPS6KA5 and EGF. HeLa cells were stained with anti-RPS6KA5 rabbit purified polyclonal antibody 1:1200 and anti-EGF mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Supplied Product
Antibody pair set content:
1. RPS6KA5 rabbit purified polyclonal antibody (100 ug)
2. EGF mouse monoclonal antibody (40 ug)
*Reagents are sufficient for at least 30-50 assays using recommended protocols.Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
In situ Proximity Ligation Assay (Cell)
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Gene Info — EGF
Entrez GeneID
1950Gene Name
EGF
Gene Alias
HOMG4, URG
Gene Description
epidermal growth factor (beta-urogastrone)
Omim ID
131530Gene Ontology
HyperlinkGene Summary
Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. [provided by RefSeq
Other Designations
urogastrone
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Gene Info — RPS6KA5
Entrez GeneID
9252Gene Name
RPS6KA5
Gene Alias
MGC1911, MSK1, MSPK1, RLPK
Gene Description
ribosomal protein S6 kinase, 90kDa, polypeptide 5
Omim ID
603607Gene Ontology
HyperlinkGene Summary
90kDa
Other Designations
mitogen- and stress-activated protein kinase 1|ribosomal protein S6 kinase, 90kD, polypeptide 5
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Interactome
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Pathway
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Disease
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