RUNX1T1 & HDAC1 Protein Protein Interaction Antibody Pair
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More Files
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In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between RUNX1T1 and HDAC1. A-549 cells were stained with anti-RUNX1T1 rabbit purified polyclonal antibody 1:100 and anti-HDAC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Representative image of Proximity Ligation Assay of protein-protein interactions between RUNX1T1 and HDAC1. HeLa cells were stained with anti-RUNX1T1 rabbit purified polyclonal antibody 1:1200 and anti-HDAC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
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Specification
Product Description
This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the RUNX1T1 protein, and the other against the HDAC1 protein for use in in situ Proximity Ligation Assay. See Publication Reference below.
Reactivity
Human
Quality Control Testing
Protein protein interaction immunofluorescence result.
Representative image of Proximity Ligation Assay of protein-protein interactions between RUNX1T1 and HDAC1. HeLa cells were stained with anti-RUNX1T1 rabbit purified polyclonal antibody 1:1200 and anti-HDAC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Supplied Product
Antibody pair set content:
1. RUNX1T1 rabbit purified polyclonal antibody (100 ug)
2. HDAC1 mouse monoclonal antibody (40 ug)
*Reagents are sufficient for at least 30-50 assays using recommended protocols.Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between RUNX1T1 and HDAC1. A-549 cells were stained with anti-RUNX1T1 rabbit purified polyclonal antibody 1:100 and anti-HDAC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue). -
Gene Info — RUNX1T1
Entrez GeneID
862Gene Name
RUNX1T1
Gene Alias
AML1T1, CBFA2T1, CDR, ETO, MGC2796, MTG8, MTG8b, ZMYND2
Gene Description
runt-related transcription factor 1; translocated to, 1 (cyclin D-related)
Omim ID
133435Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Several transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq
Other Designations
acute myelogenous leukemia 1 translocation 1 protein|acute myelogenous leukemia 1 translocation 1, cyclin-D related|core-binding factor, runt domain, alpha subunit 2; translocated to, 1; cyclin D-related|eight twenty one protein|myeloid translocation gene
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Gene Info — HDAC1
Entrez GeneID
3065Gene Name
HDAC1
Gene Alias
DKFZp686H12203, GON-10, HD1, RPD3, RPD3L1
Gene Description
histone deacetylase 1
Omim ID
601241Gene Ontology
HyperlinkGene Summary
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq
Other Designations
OTTHUMP00000008745|reduced potassium dependency, yeast homolog-like 1
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Interactome
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Pathway
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Disease
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