MAPK12 & DUSP1 Protein Protein Interaction Antibody Pair
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Specification
Product Description
This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the MAPK12 protein, and the other against the DUSP1 protein for use in in situ Proximity Ligation Assay. See Publication Reference below.
Reactivity
Human
Quality Control Testing
Protein protein interaction immunofluorescence result.
Representative image of Proximity Ligation Assay of protein-protein interactions between MAPK12 and DUSP1. HeLa cells were stained with anti-MAPK12 rabbit purified polyclonal antibody 1:1200 and anti-DUSP1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Supplied Product
Antibody pair set content:
1. MAPK12 rabbit purified polyclonal antibody (100 ug)
2. DUSP1 mouse monoclonal antibody (40 ug)
*Reagents are sufficient for at least 30-50 assays using recommended protocols.Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
In situ Proximity Ligation Assay (Cell)
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Gene Info — DUSP1
Entrez GeneID
1843Gene Name
DUSP1
Gene Alias
CL100, HVH1, MKP-1, MKP1, PTPN10
Gene Description
dual specificity phosphatase 1
Omim ID
600714Gene Ontology
HyperlinkGene Summary
The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation. [provided by RefSeq
Other Designations
serine/threonine specific protein phosphatase
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Gene Info — MAPK12
Entrez GeneID
6300Gene Name
MAPK12
Gene Alias
ERK3, ERK6, P38GAMMA, PRKM12, SAPK-3, SAPK3
Gene Description
mitogen-activated protein kinase 12
Omim ID
602399Gene Ontology
HyperlinkGene Summary
Activation of members of the mitogen-activated protein kinase family is a major mechanism for transduction of extracellular signals. Stress-activated protein kinases are one subclass of MAP kinases. The protein encoded by this gene functions as a signal transducer during differentiation of myoblasts to myotubes. [provided by RefSeq
Other Designations
mitogen-activated protein kinase 3|stress-activated protein kinase 3
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Interactome
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Pathway
- Amyotrophic lateral sclerosis (ALS)
- Epithelial cell signaling in Helicobacter pylori infection
- Fc epsilon RI signaling pathway
- GnRH signaling pathway
- Leukocyte transendothelial migration
- MAPK signaling pathway
- MAPK signaling pathway
- Neurotrophin signaling pathway
- T cell receptor signaling pathway
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