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APPL1 & TRAF2 Protein Protein Interaction Antibody Pair

  • Catalog # : DI0440
  • Visit Frequency :
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  • Specification
  • Reactivity:
  • Human
  • Quality Control Testing:
  • Protein protein interaction immunofluorescence result.

    QC Testing of DI0440
    Representative image of Proximity Ligation Assay of protein-protein interactions between APPL1 and TRAF2. HeLa cells were stained with anti-APPL1 rabbit purified polyclonal antibody 1:1200 and anti-TRAF2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
  • Supplied Product:
  • Antibody pair set content:
    1. APPL1 rabbit purified polyclonal antibody (20 ug)
    2. TRAF2 mouse monoclonal antibody (40 ug)
    *Reagents are sufficient for at least 30-50 assays using recommended protocols.
  • Storage Instruction:
  • Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
  • Publication Reference
  • Applications
  • In situ Proximity Ligation Assay (Cell)
  • Application Image
  • In situ Proximity Ligation Assay (Cell)
  • Gene Information
  • Gene Name:
  • APPL1
  • Gene Alias:
  • APPL,DIP13alpha
  • Gene Description:
  • adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
  • Gene Summary:
  • The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq
  • Other Designations:
  • AKT2 interactor,adaptor protein containing pH domain, PTB domain and leucine zipper motif 1,signaling adaptor protein DIP13alpha
  • Gene Information
  • Entrez GeneID:
  • 7186
  • Gene Name:
  • TRAF2
  • Gene Alias:
  • MGC:45012,TRAP,TRAP3
  • Gene Description:
  • TNF receptor-associated factor 2
  • Gene Summary:
  • The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs), and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of only one transcript has been determined. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000022625,OTTHUMP00000064745,tumor necrosis factor type 2 receptor associated protein 3
  • Interactome
  • Interactome
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