PIK3R1 & SHC1 Protein Protein Interaction Antibody Pair
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Specification
Product Description
This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the PIK3R1 protein, and the other against the SHC1 protein for use in in situ Proximity Ligation Assay. See Publication Reference below.
Reactivity
Human
Quality Control Testing
Protein protein interaction immunofluorescence result.
Representative image of Proximity Ligation Assay of protein-protein interactions between PIK3R1 and SHC1. HeLa cells were stained with anti-PIK3R1 rabbit purified polyclonal antibody 1:1200 and anti-SHC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Supplied Product
Antibody pair set content:
1. PIK3R1 rabbit purified polyclonal antibody (100 ug)
2. SHC1 mouse monoclonal antibody (40 ug)
*Reagents are sufficient for at least 30-50 assays using recommended protocols.Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between PIK3R1 and SHC1. Huh7 cells were stained with anti-PIK3R1 rabbit purified polyclonal antibody 1:1200 and anti-SHC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue). -
Gene Info — PIK3R1
Entrez GeneID
5295Gene Name
PIK3R1
Gene Alias
GRB1, p85, p85-ALPHA
Gene Description
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
Omim ID
171833Gene Ontology
HyperlinkGene Summary
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)|phosphatidylinositol 3-kinase, regulatory, 1|phosphatidylinositol 3-kinase-associated p-85 alpha|phosphoinositide-3-kinase, regulatory subunit 1 (p85 alpha)|phosphoinositide-3-ki
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Gene Info — SHC1
Entrez GeneID
6464Gene Name
SHC1
Gene Alias
FLJ26504, SHC, SHCA
Gene Description
SHC (Src homology 2 domain containing) transforming protein 1
Omim ID
600560Gene Ontology
HyperlinkOther Designations
OTTHUMP00000035409|OTTHUMP00000035471|SHC (Src homology 2 domain-containing) transforming protein 1|SHC-transforming protein 1
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Interactome
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Disease
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