This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the PDGFRB protein, and the other against the CRKL protein for use in in situ Proximity Ligation Assay. See Publication Reference below.
Reactivity:
Human
Quality Control Testing:
Protein protein interaction immunofluorescence result.
Representative image of Proximity Ligation Analysis of protein-protein interactions between PDGFRB and CRKL. HeLa cells were stained with anti-PDGFRB rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Supplied Product:
Antibody pair set content: 1. PDGFRB rabbit purified polyclonal antibody (100 ug) 2. CRKL mouse monoclonal antibody (40 ug) *Reagents are sufficient for at least 30-50 assays using recommended protocols.
Storage Instruction:
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
Representative image of Proximity Ligation Assay of protein-protein interactions between PDGFRB and CRKL. PC-3 cells were stained with anti-PDGFRB rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between PDGFRB and CRKL. A-549 cells were stained with anti-PDGFRB rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between PDGFRB and CRKL. Mahlavu cells were stained with anti-PDGFRB rabbit purified polyclonal antibody 1:1200 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between PDGFRB and CRKL. HT-29 cells were stained with anti-PDGFRB rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
In situ Proximity Ligation Assay (Cell)
Confocal microscopy image of Proximity Ligation Assay of protein-protein interactions between PDGFRB and CRKL. PC-3 cells were stained with anti-PDGFRB rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
In situ Proximity Ligation Assay (Cell)
Confocal microscopy image of Proximity Ligation Assay of protein-protein interactions between PDGFRB and CRKL. A-549 cells were stained with anti-PDGFRB rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
In situ Proximity Ligation Assay (Cell)
Confocal microscopy image of Proximity Ligation Assay of protein-protein interactions between PDGFRB and CRKL. HT-29 cells were stained with anti-PDGFRB rabbit purified polyclonal antibody 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia. [provided by RefSeq
This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic