AKT1 & XIAP Protein Protein Interaction Antibody Pair
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the AKT1 protein, and the other against the XIAP protein for use in in situ Proximity Ligation Assay. See Publication Reference below.
Reactivity
Human
Quality Control Testing
Protein protein interaction immunofluorescence result.
Representative image of Proximity Ligation Analysis of protein-protein interactions between AKT1 and XIAP. HeLa cells were stained with anti-AKT1 rabbit purified polyclonal antibody 1:100 and anti-XIAP mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Supplied Product
Antibody pair set content:
1. AKT1 rabbit purified polyclonal antibody (100 ug)
2. XIAP mouse monoclonal antibody (40 ug)
*Reagents are sufficient for at least 30-50 assays using recommended protocols.Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
-
Applications
In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between AKT1 and XIAP. HT-29 cells were stained with anti-AKT1 rabbit purified polyclonal antibody 1:100 and anti-XIAP mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between AKT1 and XIAP. PC-3 cells were stained with anti-AKT1 rabbit purified polyclonal antibody 1:100 and anti-XIAP mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between AKT1 and XIAP. A-549 cells were stained with anti-AKT1 rabbit purified polyclonal antibody 1:100 and anti-XIAP mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).In situ Proximity Ligation Assay (Cell)
Confocal microscopy image of Proximity Ligation Assay of protein-protein interactions between AKT1 and XIAP. HT-29 cells were stained with anti-AKT1 rabbit purified polyclonal antibody 1:100 and anti-XIAP mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue). -
Gene Info — AKT1
Entrez GeneID
207Gene Name
AKT1
Gene Alias
AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Gene Description
v-akt murine thymoma viral oncogene homolog 1
Gene Ontology
HyperlinkGene Summary
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
Other Designations
RAC-alpha serine/threonine-protein kinase|murine thymoma viral (v-akt) oncogene homolog-1|protein kinase B|rac protein kinase alpha
-
Gene Info — XIAP
Entrez GeneID
331Gene Name
XIAP
Gene Alias
API3, BIRC4, ILP1, MIHA, XLP2
Gene Description
X-linked inhibitor of apoptosis
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of a family of proteins which inhibit apoptosis through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2. This protein inhibits apoptosis induced by menadione, a potent inducer of free radicals, and ICE. It also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. [provided by RefSeq
Other Designations
OTTHUMP00000023975|OTTHUMP00000196392|apoptosis inhibitor 3|baculoviral IAP repeat-containing 4|baculoviral IAP repeat-containing protein 4
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com