APPL1 & PIK3CA Protein Protein Interaction Antibody Pair
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Specification
Product Description
This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the APPL1 protein, and the other against the PIK3CA protein for use in in situ Proximity Ligation Assay. See Publication Reference below.
Reactivity
Human
Quality Control Testing
Protein protein interaction immunofluorescence result.
Representative image of Proximity Ligation Assay of protein-protein interactions between APPL1 and PIK3CA. HeLa cells were stained with anti-APPL1 rabbit purified polyclonal antibody 1:1200 and anti-PIK3CA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Supplied Product
Antibody pair set content:
1. APPL1 rabbit purified polyclonal antibody (100 ug)
2. PIK3CA mouse monoclonal antibody (40 ug)
*Reagents are sufficient for at least 30-50 assays using recommended protocols.Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
In situ Proximity Ligation Assay (Cell)
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Gene Info — PIK3CA
Entrez GeneID
5290Gene Name
PIK3CA
Gene Alias
MGC142161, MGC142163, PI3K, p110-alpha
Gene Description
phosphoinositide-3-kinase, catalytic, alpha polypeptide
Gene Ontology
HyperlinkGene Summary
Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. [provided by RefSeq
Other Designations
PI3-kinase p110 subunit alpha|PtdIns-3-kinase p110|phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha|phosphatidylinositol 3-kinase, catalytic, alpha polypeptide|phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform
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Gene Info — APPL1
Entrez GeneID
26060Gene Name
APPL1
Gene Alias
APPL, DIP13alpha
Gene Description
adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
Omim ID
604299Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq
Other Designations
AKT2 interactor|adaptor protein containing pH domain, PTB domain and leucine zipper motif 1|signaling adaptor protein DIP13alpha
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Interactome
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Pathway
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Disease
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