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Last updated: 2017/6/25
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AKT1 & FOXO3 Protein Protein Interaction Antibody Pair

  • Catalog # : DI0289
  • Visit Frequency :
  • Countries :
  • Specification
  • Reactivity:
  • Human
  • Quality Control Testing:
  • Protein protein interaction immunofluorescence result.

    QC Testing of DI0289
    Representative image of Proximity Ligation Analysis of protein-protein interactions between AKT1 and FOXO3. HeLa cells were stained with anti-AKT1 rabbit purified polyclonal antibody 1:100 and anti-FOXO3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
  • Supplied Product:
  • Antibody pair set content:
    1. AKT1 rabbit purified polyclonal antibody (20 ug)
    2. FOXO3 mouse monoclonal antibody (40 ug)
    *Reagents are sufficient for at least 30-50 assays using recommended protocols.
  • Storage Instruction:
  • Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
  • Publication Reference
  • Applications
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • Representative image of Proximity Ligation Assay of protein-protein interactions between AKT1 and FOXO3. PC-3 cells were stained with anti-AKT1 rabbit purified polyclonal antibody 1:100 and anti-FOXO3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • Representative image of Proximity Ligation Assay of protein-protein interactions between AKT1 and FOXO3. A-549 cells were stained with anti-AKT1 rabbit purified polyclonal antibody 1:100 and anti-FOXO3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • Representative image of Proximity Ligation Assay of protein-protein interactions between AKT1 and FOXO3. HT-29 cells were stained with anti-AKT1 rabbit purified polyclonal antibody 1:100 and anti-FOXO3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • Confocal microscopy image of Proximity Ligation Assay of protein-protein interactions between AKT1 and FOXO3. PC-3 cells were stained with anti-AKT1 rabbit purified polyclonal antibody 1:100 and anti-FOXO3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • Confocal microscopy image of Proximity Ligation Assay of protein-protein interactions between AKT1 and FOXO3. HT-29 cells were stained with anti-AKT1 rabbit purified polyclonal antibody 1:100 and anti-FOXO3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
  • Application Image
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • enlarge
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • enlarge
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • enlarge
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • enlarge
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 207
  • Gene Name:
  • AKT1
  • Gene Alias:
  • AKT,MGC99656,PKB,PKB-ALPHA,PRKBA,RAC,RAC-ALPHA
  • Gene Description:
  • v-akt murine thymoma viral oncogene homolog 1
  • Gene Summary:
  • The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
  • Other Designations:
  • RAC-alpha serine/threonine-protein kinase,murine thymoma viral (v-akt) oncogene homolog-1,protein kinase B,rac protein kinase alpha
  • Gene Information
  • Entrez GeneID:
  • 2309
  • Gene Name:
  • FOXO3
  • Gene Alias:
  • AF6q21,DKFZp781A0677,FKHRL1,FKHRL1P2,FOXO2,FOXO3A,MGC12739,MGC31925
  • Gene Description:
  • forkhead box O3
  • Gene Summary:
  • This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000016944,forkhead box O3A,forkhead homolog (rhabdomyosarcoma) like 1,forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
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