CDKN1A & MDM2 Protein Protein Interaction Antibody Pair
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Specification
Product Description
This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the CDKN1A protein, and the other against the MDM2 protein for use in in situ Proximity Ligation Assay. See Publication Reference below.
Reactivity
Human
Quality Control Testing
Protein protein interaction immunofluorescence result.
Representative image of Proximity Ligation Assay of protein-protein interactions between CDKN1A and MDM2. HeLa cells were stained with anti-CDKN1A rabbit purified polyclonal antibody 1:1200 and anti-MDM2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Supplied Product
Antibody pair set content:
1. CDKN1A rabbit purified polyclonal antibody (100 ug)
2. MDM2 mouse monoclonal antibody (40 ug)
*Reagents are sufficient for at least 30-50 assays using recommended protocols.Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
In situ Proximity Ligation Assay (Cell)
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Gene Info — CDKN1A
Entrez GeneID
1026Gene Name
CDKN1A
Gene Alias
CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1
Gene Description
cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Omim ID
116899Gene Ontology
HyperlinkGene Summary
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. [provided by RefSeq
Other Designations
CDK-interaction protein 1|DNA synthesis inhibitor|OTTHUMP00000016298|cyclin-dependent kinase inhibitor 1A|melanoma differentiation associated protein 6|wild-type p53-activated fragment 1
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Gene Info — MDM2
Entrez GeneID
4193Gene Name
MDM2
Gene Alias
HDMX, MGC71221, hdm2
Gene Description
Mdm2 p53 binding protein homolog (mouse)
Omim ID
164785Gene Ontology
HyperlinkGene Summary
This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues. [provided by RefSeq
Other Designations
Mdm2, transformed 3T3 cell double minute 2, p53 binding protein|double minute 2, human homolog of; p53-binding protein|mouse double minute 2 homolog|p53-binding protein MDM2|ubiquitin-protein ligase E3 Mdm2
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Interactome
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Pathway
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Disease
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