CXCR5 & GNAI2 Protein Protein Interaction Antibody Pair
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More Files
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Representative image of Proximity Ligation Assay of protein-protein interactions between CXCR5 and GNAI2. HeLa cells were stained with anti-CXCR5 rabbit purified polyclonal antibody 1:1200 and anti-GNAI2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
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Specification
Product Description
This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the CXCR5 protein, and the other against the GNAI2 protein for use in in situ Proximity Ligation Assay. See Publication Reference below.
Reactivity
Human
Quality Control Testing
Protein protein interaction immunofluorescence result.
Representative image of Proximity Ligation Assay of protein-protein interactions between CXCR5 and GNAI2. HeLa cells were stained with anti-CXCR5 rabbit purified polyclonal antibody 1:1200 and anti-GNAI2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Supplied Product
Antibody pair set content:
1. CXCR5 rabbit purified polyclonal antibody (100 ug)
2. GNAI2 mouse monoclonal antibody (40 ug)
*Reagents are sufficient for at least 30-50 assays using recommended protocols.Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
In situ Proximity Ligation Assay (Cell)
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Gene Info — CXCR5
Entrez GeneID
643Gene Name
CXCR5
Gene Alias
BLR1, CD185, MDR15, MGC117347
Gene Description
chemokine (C-X-C motif) receptor 5
Omim ID
601613Gene Ontology
HyperlinkGene Summary
This gene was identified as a gene specifically expressed in Burkitt's lymphoma and lymphatic tissues. The protein encoded by this gene is predicted to be a seven transmembrane G protein- coupled receptor and belongs to the CXC chemokine receptor family. BLC, a B-lymphocyte chemoattractant, was identified to be a specific ligand for this receptor. Studies of this gene and its mouse conterpart strongly suggest the essential function of this gene in B cell migration and localization within specific anatomic compartments, such as follicles in lymph nodes as well as in spleen. Two alternatively spliced variants of this gene exist. [provided by RefSeq
Other Designations
Burkitt lymphoma receptor 1|Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif) receptor 5)|Burkitt lymphoma receptor 1, GTP-binding protein|C-X-C chemokine receptor type 5|monocyte-derived receptor 15
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Gene Info — GNAI2
Entrez GeneID
2771Gene Name
GNAI2
Gene Alias
GIP, GNAI2B, H_LUCA15.1, H_LUCA16.1
Gene Description
guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
Gene Ontology
HyperlinkGene Summary
O
Other Designations
GTP-binding regulatory protein Gi alpha-2 chain|WUGSC:H_LUCA15.1|WUGSC:H_LUCA16.1
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Interactome
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Pathway
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Disease
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