MAPK12 & DLG1 Protein Protein Interaction Antibody Pair
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Specification
Product Description
This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the MAPK12 protein, and the other against the DLG1 protein for use in in situ Proximity Ligation Assay. See Publication Reference below.
Reactivity
Human
Quality Control Testing
Protein protein interaction immunofluorescence result.
Representative image of Proximity Ligation Assay of protein-protein interactions between MAPK12 and DLG1. HeLa cells were stained with anti-MAPK12 rabbit purified polyclonal antibody 1:1200 and anti-DLG1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Supplied Product
Antibody pair set content:
1. MAPK12 rabbit purified polyclonal antibody (100 ug)
2. DLG1 mouse monoclonal antibody (40 ug)
*Reagents are sufficient for at least 30-50 assays using recommended protocols.Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
In situ Proximity Ligation Assay (Cell)
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Gene Info — DLG1
Entrez GeneID
1739Gene Name
DLG1
Gene Alias
DKFZp761P0818, DKFZp781B0426, DLGH1, SAP-97, SAP97, dJ1061C18.1.1, hdlg
Gene Description
discs, large homolog 1 (Drosophila)
Omim ID
601014Gene Ontology
HyperlinkGene Summary
O
Other Designations
OTTHUMP00000165202|discs large homolog 1|discs, large homolog 1|presynaptic protein SAP97|synapse-associated protein 97
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Gene Info — MAPK12
Entrez GeneID
6300Gene Name
MAPK12
Gene Alias
ERK3, ERK6, P38GAMMA, PRKM12, SAPK-3, SAPK3
Gene Description
mitogen-activated protein kinase 12
Omim ID
602399Gene Ontology
HyperlinkGene Summary
Activation of members of the mitogen-activated protein kinase family is a major mechanism for transduction of extracellular signals. Stress-activated protein kinases are one subclass of MAP kinases. The protein encoded by this gene functions as a signal transducer during differentiation of myoblasts to myotubes. [provided by RefSeq
Other Designations
mitogen-activated protein kinase 3|stress-activated protein kinase 3
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Interactome
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Pathway
- Amyotrophic lateral sclerosis (ALS)
- Epithelial cell signaling in Helicobacter pylori infection
- Fc epsilon RI signaling pathway
- GnRH signaling pathway
- Leukocyte transendothelial migration
- MAPK signaling pathway
- Neurotrophin signaling pathway
- T cell receptor signaling pathway
- T cell receptor signaling pathway
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