Protein protein interaction immunofluorescence result.
Representative image of Proximity Ligation Assay of protein-protein interactions between SMURF1 and ECSIT. HeLa cells were stained with anti-SMURF1 rabbit purified polyclonal antibody 1:1200 and anti-ECSIT mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Antibody pair set content: 1. SMURF1 rabbit purified polyclonal antibody (20 ug) 2. ECSIT mouse monoclonal antibody (40 ug) *Reagents are sufficient for at least 30-50 assays using recommended protocols.
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. A similar protein in Xenopus is involved in embryonic pattern formation. Alternative splicing results in multiple transcript variants encoding different isoforms. An additional transcript variant has been identified, but its full length sequence has not been determined. [provided by RefSeq
evolutionarily conserved signaling intermediate in Toll pathway,likely ortholog of mouse signaling intermediate in Toll pathway evolutionarily conserved,signaling intermediate in Toll pathway evolutionarily conserved ortholog,signaling intermediate in Tol