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BID & BCL2L1 Protein Protein Interaction Antibody Pair

  • Catalog # : DI0072
  • Visit Frequency :
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  • Specification
  • Reactivity:
  • Human
  • Quality Control Testing:
  • Protein protein interaction immunofluorescence result.

    QC Testing of DI0072
    Representative image of Proximity Ligation Assay of protein-protein interactions between BID and BCL2L1. HeLa cells were stained with anti-BID rabbit purified polyclonal antibody 1:1200 and anti-BCL2L1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
  • Supplied Product:
  • Antibody pair set content:
    1. BID rabbit purified polyclonal antibody (20 ug)
    2. BCL2L1 mouse monoclonal antibody (40 ug)
    *Reagents are sufficient for at least 30-50 assays using recommended protocols.
  • Storage Instruction:
  • Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
  • Publication Reference
  • Applications
  • In situ Proximity Ligation Assay (Cell)
  • Application Image
  • In situ Proximity Ligation Assay (Cell)
  • Gene Information
  • Entrez GeneID:
  • 637
  • Gene Name:
  • BID
  • Gene Alias:
  • FP497,MGC15319,MGC42355
  • Gene Description:
  • BH3 interacting domain death agonist
  • Gene Summary:
  • This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq
  • Other Designations:
  • BH3-interacting domain death agonist,BID isoform ES(1b),BID isoform L(2),BID isoform Si6,Human BID coding sequence,OTTHUMP00000196197,apoptic death agonist,desmocollin type 4
  • Gene Information
  • Entrez GeneID:
  • 598
  • Gene Name:
  • BCL2L1
  • Gene Alias:
  • BCL-XL/S,BCL2L,BCLX,Bcl-X,DKFZp781P2092,bcl-xL,bcl-xS
  • Gene Description:
  • BCL2-like 1
  • Gene Summary:
  • The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000030550,OTTHUMP00000030551,OTTHUMP00000030553
  • Interactome 1
  • Interactome
  • Interactome 2
  • Interactome
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