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PKM2 & ARAF Protein Protein Interaction Antibody Pair 

  • Catalog # : DI0063
  • Visit Frequency :
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  • Specification
  • Reactivity:
  • Human
  • Quality Control Testing:
  • Protein protein interaction immunofluorescence result.

    QC Testing of DI0063
    Representative image of Proximity Ligation Assay of protein-protein interactions between PKM2 and ARAF. HeLa cells were stained with anti-PKM2 rabbit purified polyclonal antibody 1:1200 and anti-ARAF mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
  • Supplied Product:
  • Antibody pair set content:
    1. PKM2 rabbit purified polyclonal antibody (100 ug)
    2. ARAF mouse monoclonal antibody (40 ug)
    *Reagents are sufficient for at least 30-50 assays using recommended protocols.
  • Storage Instruction:
  • Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
  • Publication Reference
  • Applications
  • In situ Proximity Ligation Assay (Cell)
  • Application Image
  • In situ Proximity Ligation Assay (Cell)
  • Gene Information
  • Entrez GeneID:
  • 5315
  • Gene Name:
  • PKM2
  • Gene Alias:
  • CTHBP,MGC3932,OIP3,PK3,PKM,TCB,THBP1
  • Gene Description:
  • pyruvate kinase, muscle
  • Gene Summary:
  • This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq
  • Other Designations:
  • OPA-interacting protein 3,PK, muscle type,pyruvate kinase M2,thyroid hormone-binding protein, cytosolic
  • Gene Information
  • Entrez GeneID:
  • 369
  • Gene Name:
  • ARAF
  • Gene Alias:
  • A-RAF,ARAF1,PKS2,RAFA1
  • Gene Description:
  • v-raf murine sarcoma 3611 viral oncogene homolog
  • Other Designations:
  • OTTHUMP00000023212,Oncogene ARAF1,Ras-binding protein DA-Raf,v-raf murine sarcoma 3611 viral oncogene homolog 1
  • Interactome 1
  • Interactome
  • Interactome 2
  • Interactome
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