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- Home > Products > Ab Pairs/Kits > Ab Pairs for PPI > TRAF2 & ACTG1 Protein Protein Interaction Antibody Pair
TRAF2 & ACTG1 Protein Protein Interaction Antibody Pair
- Catalog # : DI0041
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- 1 Set
- USD $ 535
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- Specification
- Product Description:
- This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the TRAF2 protein, and the other against the ACTG1 protein for use in in situ Proximity Ligation Assay. See Publication Reference below.
- Reactivity:
- Human
- Quality Control Testing:
- Protein protein interaction immunofluorescence result.
Representative image of Proximity Ligation Assay of protein-protein interactions between TRAF2 and ACTG1. HeLa cells were stained with anti-TRAF2 rabbit purified polyclonal antibody 1:1200 and anti-ACTG1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
- Supplied Product:
- Antibody pair set content:
1. TRAF2 rabbit purified polyclonal antibody (20 ug)
2. ACTG1 mouse monoclonal antibody (40 ug)
*Reagents are sufficient for at least 30-50 assays using recommended protocols.
- Storage Instruction:
- Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
- MSDS:
Download
- 1.
- An analysis of protein-protein interactions in cross-talk pathways reveals CRKL as a novel prognostic marker in hepatocellular carcinoma.
Liu CH, Chen TC, Chau GY, Jan YH, Chen CH, Hsu CN, Lin KT, Juang YL, Lu PJ, Cheng HC, Chen MH, Chang CF, Ting YS, Kao CY, Hsiao M, Huang CY. Mol Cell Proteomics. 2013 Feb 8. [Epub ahead of print]
- Application Image
- In situ Proximity Ligation Assay (Cell)
- Entrez GeneID:
- 7186
- Gene Name:
- TRAF2
- Gene Alias:
- MGC:45012,TRAP,TRAP3
- Gene Description:
- TNF receptor-associated factor 2
- Omim ID:
- 601895
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs), and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of only one transcript has been determined. [provided by RefSeq
- Other Designations:
- OTTHUMP00000022625,OTTHUMP00000064745,tumor necrosis factor type 2 receptor associated protein 3
- Entrez GeneID:
- 71
- Gene Name:
- ACTG1
- Gene Alias:
- ACT,ACTG,DFNA20,DFNA26
- Gene Description:
- actin, gamma 1
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells. [provided by RefSeq
- Other Designations:
- actin, cytoplasmic 2,actin, gamma 1 propeptide,cytoskeletal gamma-actin
- Interactome 1
- Interactome 2
