HCK & PIK3CB Protein Protein Interaction Antibody Pair
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Specification
Product Description
This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the HCK protein, and the other against the PIK3CB protein for use in in situ Proximity Ligation Assay. See Publication Reference below.
Reactivity
Human
Quality Control Testing
Protein protein interaction immunofluorescence result.
Representative image of Proximity Ligation Assay of protein-protein interactions between HCK and PIK3CB. HeLa cells were stained with anti-HCK rabbit purified polyclonal antibody 1:1200 and anti-PIK3CB mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Supplied Product
Antibody pair set content:
1. HCK rabbit purified polyclonal antibody (100 ug)
2. PIK3CB mouse monoclonal antibody (40 ug)
*Reagents are sufficient for at least 30-50 assays using recommended protocols.Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
In situ Proximity Ligation Assay (Cell)
Representative image of Proximity Ligation Assay of protein-protein interactions between HCK and PIK3CB. Huh7 cells were stained with anti-HCK rabbit purified polyclonal antibody 1:1200 and anti-PIK3CB mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue). -
Gene Info — HCK
Entrez GeneID
3055Gene Name
HCK
Gene Alias
JTK9
Gene Description
hemopoietic cell kinase
Omim ID
142370Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a protein-tyrosine kinase that is predominantly expressed in hemopoietic cell types. The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization. [provided by RefSeq
Other Designations
tyrosine protein kinase HCK
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Gene Info — PIK3CB
Entrez GeneID
5291Gene Name
PIK3CB
Gene Alias
DKFZp779K1237, MGC133043, PI3K, PI3KCB, PI3Kbeta, PIK3C1, p110-BETA
Gene Description
phosphoinositide-3-kinase, catalytic, beta polypeptide
Omim ID
602925Gene Ontology
HyperlinkGene Summary
Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. They have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. PI3Ks are composed of a 110-kD catalytic subunit, such as PIK3CB, and an 85-kD adaptor subunit (Hu et al., 1993 [PubMed 8246984]).[supplied by OMIM
Other Designations
PI3-kinase p110 subunit beta|PtdIns-3-kinase p110|phosphatidylinositol 3-kinase, catalytic, beta polypeptide
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Interactome
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Disease
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