2 x Taq PCR Mix with MgCl2
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Specification
Product Description
The 2 x Taq PCR Mix with MgCl2 is a premixed solution containing everything needed for a successful PCR reaction except specific primers and DNA templates. The mix includes high-quality recombinant Taq DNA polymerase, nucleotides, and magnesium in a PCR reaction buffer. For the reaction set-up add the PCR Mix (10 or 25 uL) to the primers, template, and water for the total reaction volume of 20 or 50 uL.
Amount
100 rxn (1 mL)
Quality Control Testing
Activity and stability tested at 20, 30 and 40 cycles of PCR reactions at 95°C. Free of detectable, non-specific nucleases.
Several cycles of freezing/thawing are allowed.Supplied Product
Taq DNA Polymerase (0.1 U/uL) in reaction buffer, 4 mM MgCl2, 0.4 mM of each dNTPs (dATP, dCTP, dGTP, dTTP)
Water (nuclease free) - 1 mLStorage Instruction
Store at -20°C.
Note
The mixture contains 100 units of Taq DNA Polymerase. It is sufficient for 100 reactions of 20 uL or 40 reactions of 50 uL.
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Applications
Polymerase Chain Reaction
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