In addition to 5'-3' DNA polymerase activity, it also possesses 3'-5' exonuclease (proofreading) activity. Pfu DNA Polymerase exhibits the lowest error rate of any thermostable DNA polymerase studied, is even up to ten fold more accurate than normal Taq DNA polymerase. Consequently, Pfu DNA Polymerase is useful for polymerization reactions requiring high-fidelity synthesis.
Quality Control Testing:
Free of detectable, non-specific nucleases.
10x Pfu buffer 10x Pfu Buffer with MgSO4 25 mM MgSO4
20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol.
Store at -20°C. Guaranteed stable for 6 months when properly Store.