RedTaq DNA polymerase catalyzes 5'-3' synthesis of DNA. The enzyme has been proved not having the 3'-5' exonuclease activity. The enzyme has proven to have high amplification yield, be stable at high temparetaure. Added inert dye will not have any interference to the reaction. Visual confirmation that the enzyme has been added and proper component mixing of the reaction has occurred. Samples can be loaded directly onto an agarose gel for electrophoresis with no loading dye addition.
Quality Control Testing:
Free of detectable, non-specific nucleases.
10x reaction buffer
20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol.
Store at -20°C. Guaranteed stable for 6 months when properly Store.