HotTaq DNA polymerase catalyzes 5'-3' synthesis of DNA. The enzyme has been proved not having the 3'-5' exonuclease activity. Prior the first PCR step the HotTaq DNA polymerase should be activated by 15 minute incubation at 95-97°C.
Quality Control Testing:
Free of detectable, non-specific nucleases.
10x reaction buffer
20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol.
Store at -20°C. Guaranteed stable for 6 months when properly Store.