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Last updated: 2017/9/24

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Alkaline Phosphatase (PAP)

  • Catalog # : D0009
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  • Specification
  • Product Description:
  • E.coli harboring the plasmid encoding the gene of alkaline phosphatase from Shewanella sp. SIB1 (PAP).
  • Concentration:
  • 5 units/uL
  • Quality Control Testing:
  • DNase: When 0.5 ug of lamda Hind III digest was incubated with 10 units of this enzyme in a 40 ul reaction mixture for 18 hours at 37°C no degradation of the DNA fragment is observed on agarose gel electrophoresis.
    Rnase: No RNase activity is observed by the use of RnaseAlert assay (Ambion). In this assay the reaction mixture containing the fluorescent-labeled RNA substrate was incubated with 10 units of this enzyme for 1 hours at 37°C.
  • Supplied Product:
  • Kit Content
    1.10x Alkaline Phosphatase Reaction Buffer
    2.Dilution Buffer (1x Reaction Buffer)
  • Storage Buffer:
  • 10 mM Tris-HCl (pH 7.5), 0.025 mM ZnCl, 20.25 mM MgCl2, 50% glycerol.
  • Storage Instruction:
  • Store at -20°C.
  • Note:
  • Unit Definition
    One unit is defined as the amount required to hydrolyzed 1.0 umole p-nitrophenyl phosphate per 1 minute in glycine/NaOH buffer at pH10.5 and 37°C
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