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RET Split CISH Probe 

  • Catalog # : CS0013
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  • Specification
  • Product Description:
  • RET Split CISH Probe is designed for the qualitative detection of human RET gene at 10q11.21 in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).
  • Recommend Usage:
  • The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18-25°C) and mix briefly before use.
  • Supplied Product:
  • Reagent Provided:

    1. Digoxigenin-labeled polynucleotides targeting sequences mapping in 10q11.21* (chr10:43,687,278-43,856,587) distal to the RET breakpoint region
    2. Dinitrophenyl-labeled polynucleotides targeting sequences mapping in 10q11.21* (chr10:43,340,888-43,510,171) proximal to the RET breakpoint region
    3. Formamide based hybridization buffer

    *according to Human Genome Assembly GRCh37/hg19
  • Storage Instruction:
  • Store at 2-8°C in an upright position. Return to storage conditions immediately after use.
  • Note:
  • The probe is intended to be used in combination with the CISH Implementation Kit 2 (Catalog #: KA5366), which provides necessary reagents for specimen pretreatment and post-hybridization processing.

    Hybridization signals of digoxigenin-labeled polynucleotides appear dark green colored distinct dotshaped (distal to the RET breakpoint region), and dinitrophenyl-labeled polynucleotides appear bright red distinct dot-shaped (proximal to the RET breakpoint region)).
    Normal situation: In interphases of normal cells or cells without a translocation involving the RET gene region, two red/green fusion signals appear.
    Aberrant situation: One RET gene region affected by a translocation is indicated by one separate green signal and one separate red signal. Isolated green signals are the result of deletions proximal to the RET breakpoint region. Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.
    Unexpected signal patterns should be further investigated.
  • Probe Position:
  • Regulatory Status:
  • For research use only (RUO)
  • Interpretation of Result:
  • Applications
  • Chromogenic In Situ Hybridization (Cells)
  • Chromogenic <i>In Situ</i> Hybridization (Cells)
  • Normal interphase cells as indicated by two red/green fusion signals per nucleus.
  • Application Image
  • Chromogenic In Situ Hybridization (Cells)
  • Chromogenic <i>In Situ</i> Hybridization (Cells)
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  • Gene Information
  • Entrez GeneID:
  • 5979
  • Gene Name:
  • RET
  • Gene Alias:
  • Gene Description:
  • ret proto-oncogene
  • Gene Summary:
  • This gene, a member of the cadherin superfamily, encodes one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. This gene plays a crucial role in neural crest development, and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in this gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their biological validity has not been confirmed. [provided by RefSeq
  • Other Designations:
  • RET transforming sequence,cadherin family member 12,hydroxyaryl-protein kinase,oncogene RET,receptor tyrosine kinase,ret proto-oncogene (multiple endocrine neoplasia and medullary thyroid carcinoma 1, Hirschsprung disease)
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