Products

Product Description

DDIT3 Split CISH Probe is designed for the qualitative detection of translocations involving the human DDIT3 gene at 12q13.3 in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).

Reactivity

Human

Recommend Usage

The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18-25°C) and mix briefly before use.

Supplied Product

Reagent Provided:

This Probe is composed of:
1. Digoxigenin-labeled polynucleotides, which target sequences mapping in 12q13.3-q14.1* (chr12:58,024,366-58,486,511) distal to the DDIT3 breakpoint region.
2. Dinitrophenyl-labeled polynucleotides, which target sequences mapping in 12q13.3* (chr12:57,386,302-57,865,800) proximal to the DDIT3 breakpoint region.
3. Formamide based hybridization buffer.

*according to Human Genome Assembly GRCh37/hg19

Probe Position

Regulatory Status

For research use only (RUO)

Storage Instruction

Store at 2-8°C in an upright position. Return to storage conditions immediately after use.

Note

The probe is intended to be used in combination with the CISH Implementation Kit 2 (Catalog #: KA5366), which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Interpretation of results:
Using the CISH Implementation Kit 2 (Cat # KA5366), hybridization signals of Digoxigenin-labeled polynucleotides appear as dark green colored distinct dots (distal to the DDIT3 breakpoint region), and Dinitrophenyl-labeled polynucleotides appear as bright red colored distinct dots (proximal to the DDIT3 breakpoint region).
Normal situation: In interphases of normal cells or cells without a translocation involving the DDIT3 gene region, two red/green fusion signals appear.
Aberrant situation: One DDIT3 gene region affected by a translocation is indicated by one separate distinct dot-shaped green signal and one separate distinct dot-shaped red signal.
Overlapping signals may appear as brown signals. Genomic aberrations due to small deletions, duplications or inversions might result in inconspicuous signal patterns. Other signal patterns than those described above may be observed in some abnormal samples. These unexpected signal patterns should be further investigated.

Interpretation of Result