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Last updated: 2019/8/18

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ALK Split CISH Probe 

  • Catalog # : CS0001
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  • Specification
  • Product Description:
  • ALK Split CISH Probe is designed for the qualitative detection of human ALK gene at 2p23.2 in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).
  • Recommend Usage:
  • The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18-25°C) and mix briefly before use.
  • Supplied Product:
  • Reagent Provided:

    1. Digoxigenin-labeled polynucleotides targeting sequences mapping in 2p23.2* (chr2:29,460,144-29,681,581) proximal to the ALK breakpoint region.
    2. Dinitrophenyl-labeled polynucleotides targeting sequences mapping in 2p23.2* (chr2:29,174,204-29,383,335) distal to the ALK breakpoint region.
    3. Formamide based hybridization buffer.

    *according to Human Genome Assembly GRCh37/hg19
  • Storage Instruction:
  • Store at 2-8°C in an upright position. Return to storage conditions immediately after use.
  • Note:
  • The probe is intended to be used in combination with the CISH Implementation Kit 2 (Catalog #: KA5366), which provides necessary reagents for specimen pretreatment and post-hybridization processing.

    Hybridization signals of digoxigenin-labeled polynucleotides appear dark green distinct dot-shaped (proximal to the ALK breakpoint region), and dinitrophenyl-labeled polynucleotides appear bright red distinct dot-shaped (distal to the ALK breakpoint region).
    Normal situation: In interphases of normal cells or cells without a translocation involving the ALK gene region, two red/green fusion signals appear.
    Aberrant situation: One ALK gene region affected by a translocation is indicated by one separate green signal and one separate red signal. EML4-ALK inversion with deletion of 5'-ALK sequences is indicated by one or multiple isolated red signals.
    Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.
    Unexpected signal patterns should be further investigated.
  • Probe Position:
  • Regulatory Status:
  • For research use only (RUO)
  • Interpretation of Result:
  • Applications
  • Chromogenic In Situ Hybridization (FFPE Tissue)
  • Chromogenic <i>In Situ</i> Hybridization (FFPE Tissue)
  • Lung carcinoma tissue section with translocation affecting the 2p23.2 locus as indicated by one red/green fusion (non-rearranged) signal, one red signal, and one separate green signal.
  • Application Image
  • Chromogenic In Situ Hybridization (FFPE Tissue)
  • Chromogenic <i>In Situ</i> Hybridization (FFPE Tissue)
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  • Gene Information
  • Entrez GeneID:
  • 238
  • Gene Name:
  • ALK
  • Gene Alias:
  • CD246,Ki-1,TFG/ALK
  • Gene Description:
  • anaplastic lymphoma receptor tyrosine kinase
  • Gene Summary:
  • The 2;5 chromosomal translocation is frequently associated with anaplastic large cell lymphomas (ALCLs). The translocation creates a fusion gene consisting of the ALK (anaplastic lymphoma kinase) gene and the nucleophosmin (NPM) gene: the 3' half of ALK, derived from chromosome 2, is fused to the 5' portion of NPM from chromosome 5. A recent study shows that the product of the NPM-ALK fusion gene is oncogenic. The deduced amino acid sequences reveal that ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. These sequences are absent in the product of the transforming NPM-ALK gene. ALK shows the greatest sequence similarity to LTK (leukocyte tyrosine kinase). ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. [provided by RefSeq
  • Other Designations:
  • ALK tyrosine kinase receptor,CD246 antigen,anaplastic lymphoma kinase (Ki-1),anaplastic lymphoma kinase Ki-1
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