Human lg-kappa CISH Probe
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Chromogenic In Situ Hybridization (FFPE Tissue)
CISH analysis of tonsil tissue with B-cells expressing Ig-kappa hybridized with Human lg-kappa CISH Probe
-
Specification
Product Description
Human lg-kappa CISH Probe is designed for the qualitative detection of human Ig-kappa light chain mRNA in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).
Reactivity
Human
Recommend Usage
The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (55°C) and mix briefly before use. Human lg-kappa CISH Probe is typically used in conjunction with Human Ig-lambda CISH Probe to assist in the distinction between polyclonal and monoclonal lymphoid proliferations.
Supplied Product
Reagent Provided:
Digoxigenin-labeled oligonucleotides targeting mRNA sequences encoding Ig-kappa light chain constant regions.Regulatory Status
For research use only (RUO)
Storage Instruction
Store at 2-8°C in an upright position. Return to storage conditions immediately after use.
Note
The probe is intended to be used in combination with the CISH Implementation HRP-DAB Kit (Catalog #: KA5367), which provides necessary reagents for specimen pretreatment and post-hybridization processing.
A positive reactivity in plasma B-cells is indicated by cytoplasmic staining. In lymphoid tissue, the normal kappa-to-lambda ratio is roughly 2:1, an indication for monoclonality is given if the kappa-to-lambda ratio is greater than 3:1 or less than 0.3:1. Visualization of signals should be performed at least at 100-fold magnification resulting in easily visible signals. Do not evaluate areas of necrosis, overlapping nuclei, over-digested nuclei, and nuclei with weak signal intensity. -
Applications
Chromogenic In Situ Hybridization (FFPE Tissue)
CISH analysis of tonsil tissue with B-cells expressing Ig-kappa hybridized with Human lg-kappa CISH Probe
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com