HPV type 31/33 CISH Probe
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
HPV type 31/33 CISH Probe is designed for the qualitative detection of human papillomavirus (HPV) type 31/33 DNA in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).
Reactivity
Human
Recommend Usage
The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (37°C) and mix briefly before use.
Supplied Product
Reagent Provided:
1.Digoxigenin-labeled oligonucleotides specific for HPV type 31/33, which target DNA sequences encoding for the HPV 31/33 proteins E6, E7, and/or L1. The probe also targets the respective RNA sequences of E6, E7, and/or L1 proteins, which are expressed during some stages of infection.
2. Formamide based hybridization buffer.Regulatory Status
For research use only (RUO)
Storage Instruction
Store at 2-8°C in an upright position. Return to storage conditions immediately after use.
Note
The probe is intended to be used in combination with the CISH Implementation HRP-DAB Kit (Catalog #: KA5367), which provides necessary reagents for specimen pretreatment and post-hybridization processing.
The staining pattern in the nucleus can be observed as discrete and dot-shaped signals in case of integrated HPV, or as a strong and homogeneous nuclear staining in case of episomal HPV. A cytoplasmic staining is observed when RNA sequences of HPV are detected. Visualization of signals should be performed using a set of objectives ranging from an at least 200-fold to 630-fold magnification. The presence of the episomal staining pattern is usually detected clearly by an objective with 200-fold magnification, whereas the detection of the integrated HPV pattern requires a greater magnification, preferably 630-fold. Do not evaluate areas of necrosis, overlapping nuclei, over-digested nuclei and nuclei with weak signal intensity. -
Applications
Chromogenic In Situ Hybridization (FFPE Tissue)
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com