HPV High-Risk (HR) Types CISH Probe
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Chromogenic In Situ Hybridization (FFPE Tissue)
CISH analysis of HPV infected cervix tissue hybridized with HPV High-Risk (HR) Types CISH Probe
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Specification
Product Description
HPV High-Risk (HR) Types CISH Probeis designed for the qualitative detection of human papillomavirus (HPV) type 16/18/31/33/35/39/45/51/52/56/58/59/66/68/82 DNA in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).
Reactivity
Human
Recommend Usage
The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (37°C) and mix briefly before use.
Supplied Product
Reagent Provided:
1.Dinitrophenyl-labeled polynucleotides specific for HPV type 16/18/31/33/35/39/45/51/52/56/58/59/66/68/82, which target DNA sequences encoding for the HPV 16/18/31/33/35/39/45/51/52/56/58/59/66/68/82 proteins E6, E7, and/or L1. The probe also targets the respective RNA sequences of E6, E7, and/or L1 proteins, which are expressed during some stages of infection.
2. Formamide based hybridization buffer.Regulatory Status
For research use only (RUO)
Storage Instruction
Store at 2-8°C in an upright position. Return to storage conditions immediately after use.
Note
The probe is intended to be used in combination with the CISH Implementation HRP-DAB Kit (Catalog #: KA5367), which provides necessary reagents for specimen pretreatment and post-hybridization processing.
The staining pattern in the nucleus can be observed as discrete and dot-shaped signals in case of integrated HPV, or as a strong and homogeneous nuclear staining in case of episomal HPV. A cytoplasmic staining is observed when RNA sequences of HPV are detected. -
Applications
Chromogenic In Situ Hybridization (FFPE Tissue)
CISH analysis of HPV infected cervix tissue hybridized with HPV High-Risk (HR) Types CISH Probe
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