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Last updated: 2021/1/17

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CMV CISH Probe 

  • Catalog # : CG0023
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  • Specification
  • Product Description:
  • CMV CISH Probe is designed for the qualitative detection cytomegalovirus (CMV) DNA in formalin-fixed, paraffin-embedded tissue or cells by chromogenic in situ hybridization (CISH).
  • Recommend Usage:
  • The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (37°C) and mix briefly before use.
  • Supplied Product:
  • Reagent Provided:

    1. Digoxigenin-labeled oligonucleotides targeting the sequence of β2.7 gene, the most abundantly transcribed early CMV gene.
    2. Formamide based hybridization buffer.
  • Storage Instruction:
  • Store at 2-8°C in an upright position. Return to storage conditions immediately after use.
  • Note:
  • The probe is intended to be used in combination with the CISH Implementation HRP-DAB Kit (Catalog #: KA5367), which provides necessary reagents for specimen pretreatment and post-hybridization processing.

    A positive reactivity for CMV DNA in the target cells is indicated by a distinctly stained nucleus. Visualization of signals should be performed at least at 100-fold magnification resulting in easily visible signals. Do not evaluate areas of necrosis, overlapping nuclei, over-digested nuclei, and nuclei with weak signal intensity.
  • Regulatory Status:
  • For research use only (RUO)
  • Applications
  • Chromogenic In Situ Hybridization (FFPE Tissue)
  • Chromogenic <i>In Situ</i> Hybridization (FFPE Tissue)
  • CISH analysis of paraffin-embedded adrenal gland tissue hybridized with using with CMV CISH Probe.
  • Application Image
  • Chromogenic In Situ Hybridization (FFPE Tissue)
  • Chromogenic <i>In Situ</i> Hybridization (FFPE Tissue)
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