CMV CISH Probe
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Chromogenic In Situ Hybridization (FFPE Tissue)
CISH analysis of paraffin-embedded adrenal gland tissue hybridized with using with CMV CISH Probe.
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Specification
Product Description
CMV CISH Probe is designed for the qualitative detection cytomegalovirus (CMV) DNA in formalin-fixed, paraffin-embedded tissue or cells by chromogenic in situ hybridization (CISH).
Reactivity
Human
Recommend Usage
The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (37°C) and mix briefly before use.
Supplied Product
Reagent Provided:
1. Digoxigenin-labeled oligonucleotides targeting the sequence of β2.7 gene, the most abundantly transcribed early CMV gene.
2. Formamide based hybridization buffer.Regulatory Status
For research use only (RUO)
Storage Instruction
Store at 2-8°C in an upright position. Return to storage conditions immediately after use.
Note
The probe is intended to be used in combination with the CISH Implementation HRP-DAB Kit (Catalog #: KA5367), which provides necessary reagents for specimen pretreatment and post-hybridization processing.
A positive reactivity for CMV DNA in the target cells is indicated by a distinctly stained nucleus. Visualization of signals should be performed at least at 100-fold magnification resulting in easily visible signals. Do not evaluate areas of necrosis, overlapping nuclei, over-digested nuclei, and nuclei with weak signal intensity. -
Applications
Chromogenic In Situ Hybridization (FFPE Tissue)
CISH analysis of paraffin-embedded adrenal gland tissue hybridized with using with CMV CISH Probe.
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