MYCN CISH Probe
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Specification
Product Description
MYCN CISH Probe is designed for the qualitative detection human MYCN gene in formalin-fixed, paraffin-embedded tissue or cells by chromogenic in situ hybridization (CISH).
Reactivity
Human
Recommend Usage
The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18-25°C) and mix briefly before use.
Supplied Product
Reagent Provided:
Digoxigenin-labeled oligonucleotides targeting sequences mapping in 2p24.3* (chr2:15,846,046-16,213,717) harboring the MYCN gene.
2. Formamide based hybridization buffer.
*According to Human Genome Assembly GRCh37/hg19Probe Position
Regulatory Status
For research use only (RUO)
Storage Instruction
Store at 2-8°C in an upright position. Return to storage conditions immediately after use.
Note
The probe is intended to be used in combination with the CISH Implementation Kit 2 (Catalog #: KA5366), which provides necessary reagents for specimen pretreatment and post-hybridization processing.
Hybridization signals of digoxigenin-labeled polynucleotides appear as brown- to dark brown colored distinct dots (MYCN gene).
Normal situation: In interphases of normal cells or cells without an amplification involving the MYCN gene locus, two distinct dot-shaped brown signals per nucleus appear.
Aberrant situation: In a cell with amplification of the MYCN gene locus or aneuploidy of chromosome 2, multiple copies of the brown signal or brown signal clusters will be observed.Interpretation of Result
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Applications
Chromogenic In Situ Hybridization (FFPE Tissue)
Normal nuclei each with two MYCN signals -
Gene Info — MYCN
Entrez GeneID
4613Gene Name
MYCN
Gene Alias
MODED, N-myc, NMYC, ODED, bHLHe37
Gene Description
v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)
Gene Ontology
HyperlinkGene Summary
This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. [provided by RefSeq
Other Designations
N-myc proto-oncogene protein|neuroblastoma MYC oncogene|neuroblastoma-derived v-myc avian myelocytomatosis viral related oncogene|oncogene NMYC|pp65/67|v-myc avian myelocytomatosis viral related oncogene, neuroblastoma derived|v-myc myelocytomatosis viral
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Interactome
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Disease
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