EGFR CISH Probe
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
EGFR CISH Probe is designed for the qualitative detection of human EGFR gene in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).
Reactivity
Human
Recommend Usage
The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18-25°C) and mix briefly before use.
Supplied Product
Reagent Provided:
1. Digoxigenin-labeled polynucleotides targeting sequences mapping in 7p11.2-7p12* (chr7:55,034,991-55,380,617) harboring the EGFR gene
2. Formamide based hybridization buffer
*according to Human Genome Assembly GRCh37/hg19Probe Position
Regulatory Status
For research use only (RUO)
Storage Instruction
Store at 2-8°C in an upright position. Return to storage conditions immediately after use.
Note
The probe is intended to be used in combination with the CISH Implementation Kit 1 (Catalog #: KA5365), which provides necessary reagents for specimen pretreatment and post-hybridization processing.
Hybridization signals of digoxigenin-labeled polynucleotides appear as brown- to dark brown colored distinct dots.
Normal situation: In interphases of normal cells or cells without an amplification involving the 7p11.2 band, two distinct dot-shaped brown signals per nuclei appear.
Aberrant situation: In a cell with amplification of the EGFR gene locus or aneuploidy of chromosome 7, multiple copies of the brown signal or brown signal clusters will be observed. Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.
Unexpected signal patterns should be further investigated.Interpretation of Result
-
Applications
Chromogenic In Situ Hybridization (FFPE Tissue)
Normal nuclei each with two EGFR signals.Chromogenic In Situ Hybridization (Cells)
NSCLC cancer cells with multiple EGFR signals in sputum sample. -
Gene Info — EGFR
Entrez GeneID
1956Gene Name
EGFR
Gene Alias
ERBB, ERBB1, HER1, PIG61, mENA
Gene Description
epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
Other Designations
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com