CDK4/CEN12 CISH Probe
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Chromogenic In Situ Hybridization (FFPE Tissue)
Liposarcoma tissue section with CDK4 amplification as indicated by large green clusters.
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Specification
Product Description
CDK4/CEN12 CISH Probe is designed for the qualitative detection of human CDK4 gene amplifications as well as the detection of chromosome 12 alpha satellites in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).
Reactivity
Human
Recommend Usage
The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18-25°C) and mix briefly before use.
Supplied Product
Reagent Provided:
This Probe is composed of:
1. Digoxigenin-labeled polynucleotides, which target sequences mapping in 12q13.3-q14.1* (chr12:58,004,553-58,313,271) harboring the CDK4 gene region.
2. Dinitrophenyl-labeled polynucleotides, which target sequences mapping in 12p11.1-q11 specific for the alpha satellite centromeric region D12Z3 of chromosome 12.
3. Formamide based hybridization buffer.
*according to Human Genome Assembly GRCh37/hg19Probe Position
Regulatory Status
For research use only (RUO)
Storage Instruction
Store at 2-8°C in an upright position. Return to storage conditions immediately after use.
Note
The probe is intended to be used in combination with the CISH Implementation Kit 2 (Catalog #: KA5366), which provides necessary reagents for specimen pretreatment and post-hybridization processing.
Interpretation of results:
Using the CISH Implementation Kit 2 (Cat # KA5366), hybridization signals of Digoxigenin-labeled polynucleotides appear as dark green colored distinct dots (CDKN2A gene region), and Dinitrophenyl-labeled polynucleotides appear as bright red colored distinct dots (CEN 9).
Normal situation: In interphases of normal cells or cells without a deletion involving the CDKN2A gene region, two distinct dot-shaped green and two distinct dot-shaped red signals appear.
Aberrant situation: In a cell with deletion affecting the CDKN2A gene region, a reduced number of green signals will be observed. Deletions affecting only parts of the CDKN2A gene region might result in normal signal pattern with green signals of reduced size.
Overlapping signals may appear as brown signals. Other signal patterns than those described above may be observed in some abnormal samples. These unexpected signal patterns should be further investigated.Interpretation of Result
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Applications
Chromogenic In Situ Hybridization (FFPE Tissue)
Liposarcoma tissue section with CDK4 amplification as indicated by large green clusters. -
Gene Info — CDK4
Entrez GeneID
1019Gene Name
CDK4
Gene Alias
CMM3, MGC14458, PSK-J3
Gene Description
cyclin-dependent kinase 4
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq
Other Designations
cell division kinase 4|melanoma cutaneous malignant, 3
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Interactome
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Pathway
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Disease
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