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MET (phospho Y1349/1356) polyclonal antibody   

  • Catalog # : PAB9992
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  • 100 ug
  • USD $ 465
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of MET.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding Y1349/Y1356 of human MET.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody is specific to human c-Met protein phosphorylated at Y1349 and Y1356.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:450000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using MET (phospho Y1349/1356) polyclonal antibody (Cat # PAB9992) shows detection of phosphorylated MET.
    Human mammary B5/589 epithelial cells were serum-deprived and treated with or without HGF.
    Cell lysates were immunoprecipated with the anti-MET antibody, resolved by SDS-PAGE, transferred to PVDF membrane, and probed with MET (phospho Y1349/1356) polyclonal antibody (Cat # PAB9992).
    Personal communication, D. Bottaro and T. Ito, NCI, Bethesda, MD.
  • Immunohistochemistry
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 4233
  • Protein Accession#:
  • NP_000236;P08581
  • Gene Name:
  • MET
  • Gene Alias:
  • AUTS9,HGFR,RCCP2,c-Met
  • Gene Description:
  • met proto-oncogene (hepatocyte growth factor receptor)
  • Gene Summary:
  • The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • HGF receptor,OTTHUMP00000069168,SF receptor,met proto-oncogene,met proto-oncogene tyrosine kinase,oncogene MET,scatter factor receptor
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