MET (phospho Y1349/1356) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot using MET (phospho Y1349/1356) polyclonal antibody (Cat # PAB9992) shows detection of phosphorylated MET.
Human mammary B5/589 epithelial cells were serum-deprived and treated with or without HGF.
Cell lysates were immunoprecipated with the anti-MET antibody, resolved by SDS-PAGE, transferred to PVDF membrane, and probed with MET (phospho Y1349/1356) polyclonal antibody (Cat # PAB9992).
Personal communication, D. Bottaro and T. Ito, NCI, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of MET.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Y1349/Y1356 of human MET.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to human c-Met protein phosphorylated at Y1349 and Y1356.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:450000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using MET (phospho Y1349/1356) polyclonal antibody (Cat # PAB9992) shows detection of phosphorylated MET.
Human mammary B5/589 epithelial cells were serum-deprived and treated with or without HGF.
Cell lysates were immunoprecipated with the anti-MET antibody, resolved by SDS-PAGE, transferred to PVDF membrane, and probed with MET (phospho Y1349/1356) polyclonal antibody (Cat # PAB9992).
Personal communication, D. Bottaro and T. Ito, NCI, Bethesda, MD.Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — MET
Entrez GeneID
4233Protein Accession#
NP_000236;P08581Gene Name
MET
Gene Alias
AUTS9, HGFR, RCCP2, c-Met
Gene Description
met proto-oncogene (hepatocyte growth factor receptor)
Gene Ontology
HyperlinkGene Summary
The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
HGF receptor|OTTHUMP00000069168|SF receptor|met proto-oncogene|met proto-oncogene tyrosine kinase|oncogene MET|scatter factor receptor
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Interactome
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Pathway
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Disease
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Publication Reference
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Intracellular signaling cascades triggered by the NK1 fragment of hepatocyte growth factor in human prostate epithelial cell line PNT1A.
Pavone LM, Cattaneo F, Rea S, De Pasquale V, Spina A, Sauchelli E, Mastellone V, Ammendola R.
Cellular Signalling 2011 Dec; 23(12):1961.
Application：WB-Ce, Human, PNT1A cells.
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Met, metastasis, motility and more.
Birchmeier C, Birchmeier W, Gherardi E, Vande Woude GF.
Nature Reviews. Molecular Cell Biology 2003 Dec; 4(12):915.
Application：WB, Human, Mammalian cells.
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The multisubstrate adapter Gab1 regulates hepatocyte growth factor (scatter factor)-c-Met signaling for cell survival and DNA repair.
Fan S, Ma YX, Gao M, Yuan RQ, Meng Q, Goldberg ID, Rosen EM.
Mol Cell Biol 2001 Aug; 21(15):4968.
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Receptor tyrosine kinases as therapeutic targets: the model of the MET oncogene.
Longati P, Comoglio PM, Bardelli A.
Current Drug Targets 2001 Mar; 2(1):41.
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Intracellular signaling cascades triggered by the NK1 fragment of hepatocyte growth factor in human prostate epithelial cell line PNT1A.
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