JAG1 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot using JAG1 polyclonal antibody (Cat # PAB9954) shows detection of JAG1 protein in various whole cell lysates : human brain (Lane 1), human kidney (Lane 2), human liver (Lane 3), and mouse liver (Lane 5).
Lane 4 contained sample buffer only.
The band at ~134 KDa in lane 5 is believed to be JAG1 precursor.
The identity of minor reactive bands is unknown.
Each lane contains approximately 20 ug of lysate.
Primary antibody was used at a 1 : 500 dilution.
The membrane was washed and reacted with a 1 : 5,000 dilution of HRP conjugated Gt-a-Rabbit IgG.
Exposure time was 1 min.
Predicted molecular weight is 134 KDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cervical cancer tissue (40X magnification) using JAG1 polyclonal antibody (Cat # PAB9954) . Tissue was fixed with formalin and embedded in paraffin.
Hematoxylin was used to counter-stain cells. A 1 : 100 dilution of primary antibody was used.
Personal Communication. Martin Kast Laboratory.
Immunofluorescence
Immunofluorescence Microscopy using JAG1 polyclonal antibody (Cat # PAB9954) of human corneal epithelial cells.
Primary antibody was used at a 1 : 500 dilution.
The JAG1 (green staining) is local-ized to the cytoplasm and is consistent with reports in the literature.
The nucleus is stained with Bis benzimide (blue) .
Personal Communication. Aihua Ma, Univdersity of Cardiff.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of JAG1.
Immunogen
A synthetic peptide corresponding to amino acids 110-125 of human JAG1.
Host
Rabbit
Reactivity
Clawed frog, Dog, Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:5000-1:20000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:100-1:500)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot using JAG1 polyclonal antibody (Cat # PAB9954) shows detection of JAG1 protein in various whole cell lysates : human brain (Lane 1), human kidney (Lane 2), human liver (Lane 3), and mouse liver (Lane 5).
Lane 4 contained sample buffer only.
The band at ~134 KDa in lane 5 is believed to be JAG1 precursor.
The identity of minor reactive bands is unknown.
Each lane contains approximately 20 ug of lysate.
Primary antibody was used at a 1 : 500 dilution.
The membrane was washed and reacted with a 1 : 5,000 dilution of HRP conjugated Gt-a-Rabbit IgG.
Exposure time was 1 min.
Predicted molecular weight is 134 KDa.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cervical cancer tissue (40X magnification) using JAG1 polyclonal antibody (Cat # PAB9954) . Tissue was fixed with formalin and embedded in paraffin.
Hematoxylin was used to counter-stain cells. A 1 : 100 dilution of primary antibody was used.
Personal Communication. Martin Kast Laboratory.Immunofluorescence
Immunofluorescence Microscopy using JAG1 polyclonal antibody (Cat # PAB9954) of human corneal epithelial cells.
Primary antibody was used at a 1 : 500 dilution.
The JAG1 (green staining) is local-ized to the cytoplasm and is consistent with reports in the literature.
The nucleus is stained with Bis benzimide (blue) .
Personal Communication. Aihua Ma, Univdersity of Cardiff.Enzyme-linked Immunoabsorbent Assay
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Gene Info — JAG1
Entrez GeneID
182Gene Name
JAG1
Gene Alias
AGS, AHD, AWS, CD339, HJ1, JAGL1, MGC104644
Gene Description
jagged 1 (Alagille syndrome)
Gene Ontology
HyperlinkGene Summary
The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis. [provided by RefSeq
Other Designations
OTTHUMP00000030278|jagged 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Immunohistological localization of Notch receptors and their ligands Delta and Jagged in synovial tissues of rheumatoid arthritis.
Yabe Y, Matsumoto T, Tsurumoto T, Shindo H.
Journal of Orthopaedic Science 2005 Nov; 10(6):589.
Application：IHC-P, Human, Rheumatoid arthritis synovium.
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Complementation of human papillomavirus type 16 E6 and E7 by Jagged1-specific Notch1-phosphatidylinositol 3-kinase signaling involves pleiotropic oncogenic functions independent of CBF1;Su(H);Lag-1 activation.
Veeraraghavalu K, Subbaiah VK, Srivastava S, Chakrabarti O, Syal R, Krishna S.
Journal of Virology 2005 Jun; 79(12):7889.
Application：IF, WB, Human, CaSki, HaCaT, SiHa cells.
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Crosstalk between Jagged1 and GDNF/Ret/GFRalpha1 signalling regulates ureteric budding and branching.
Kuure S, Sainio K, Vuolteenaho R, Ilves M, Wartiovaara K, Immonen T, Kvist J, Vainio S, Sariola H.
Mechanisms of Development 2005 Jun; 122(6):765.
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Immunohistological localization of Notch receptors and their ligands Delta and Jagged in synovial tissues of rheumatoid arthritis.
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