CCNB1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant CCNB1.
Immunogen
Recombinant GST fusion protein corresponding to full length human CCNB1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:2000-1:10000)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using CCNB1 polyclonal antibody (Cat # PAB9943) antibody shows detection of human CCNB1 present in asynchronous HN30 cell lysates.
HN30 cells are from head and neck cancer tumors that over express CCNB1 and D1.
Comparison to a molecular weight marker indicates a band of ~62 KDa corresponding to the expected molecular weight for the protein (arrowhead).
The blot was incubated with a 1 : 500 dilution of the antibody for 1 h at room temperature.
Detection occurred using a 1 : 10,000 of HRP conjugated Goat-a-Rabbit IgG and chemiluminescence reagent with a 1-min exposure time.
Personal communication, Luca Cote, Temple University, Philadelphia, PA.Western Blot (Cell lysate)
Western blot analysis using CCNB1 polyclonal antibody (Cat # PAB9943) shows detection of CCNB1 present in asynchronous HeLa cell lysates.
Comparison to a molecular weight marker indicates a band of ~55 KDa corresponding to human CCNB1 (arrowhead).
Approximately 50 ug of lysate was loaded on to a 7% SDS-PAGE gel for separation.
After transfer to nitrocellulose, the blot was incubated with a 1 : 500 dilution of the antibody for 1 h at room temperature.
Detection occurred using a 1 : 10,000 of HRP conjugated Goat-a-Rabbit IgG.
Personal communication, Luca D'Agostino, Temple University, Philadelphia, PA.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining with CCNB1 polyclonal antibody (Cat # PAB9943) was diluted 1 : 500 to detect CCNB1 in human brain cerebellum tissue.
Tissue was formalin fixed and paraffin embedded.
No pre-treatment of sample was required.
The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — CCNB1
Entrez GeneID
891Protein Accession#
NP_114172;P14635Gene Name
CCNB1
Gene Alias
CCNB
Gene Description
cyclin B1
Omim ID
123836Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq
Other Designations
G2/mitotic-specific cyclin B1
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Interactome
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Pathway
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Disease
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Publication Reference
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Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B.
Pines J, Hunter T.
Nature 1990 Aug; 346(6286):760.
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A 60 kd cdc2-associated polypeptide complexes with the E1A proteins in adenovirus-infected cells.
Giordano A, Whyte P, Harlow E, Franza BR Jr, Beach D, Draetta G.
Cell 1989 Sep; 58(5):981.
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Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B.
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