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Last updated: 2014/9/1
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CCNB1 polyclonal antibody

  • Catalog # : PAB9943
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against full length recombinant CCNB1.
  • Immunogen:
  • Recombinant GST fusion protein corresponding to full length human CCNB1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA (1:2000-1:10000)
    Western Blot (1:500-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In antiserum (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using CCNB1 polyclonal antibody (Cat # PAB9943) shows detection of CCNB1 present in asynchronous HeLa cell lysates.
    Comparison to a molecular weight marker indicates a band of ~55 KDa corresponding to human CCNB1 (arrowhead).
    Approximately 50 ug of lysate was loaded on to a 7% SDS-PAGE gel for separation.
    After transfer to nitrocellulose, the blot was incubated with a 1 : 500 dilution of the antibody for 1 h at room temperature.
    Detection occurred using a 1 : 10,000 of HRP conjugated Goat-a-Rabbit IgG.
    Personal communication, Luca D'Agostino, Temple University, Philadelphia, PA.
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using CCNB1 polyclonal antibody (Cat # PAB9943) antibody shows detection of human CCNB1 present in asynchronous HN30 cell lysates.
    HN30 cells are from head and neck cancer tumors that over express CCNB1 and D1.
    Comparison to a molecular weight marker indicates a band of ~62 KDa corresponding to the expected molecular weight for the protein (arrowhead).
    The blot was incubated with a 1 : 500 dilution of the antibody for 1 h at room temperature.
    Detection occurred using a 1 : 10,000 of HRP conjugated Goat-a-Rabbit IgG and chemiluminescence reagent with a 1-min exposure time.
    Personal communication, Luca Cote, Temple University, Philadelphia, PA.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining with CCNB1 polyclonal antibody (Cat # PAB9943) was diluted 1 : 500 to detect CCNB1 in human brain cerebellum tissue.
    Tissue was formalin fixed and paraffin embedded.
    No pre-treatment of sample was required.
    The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 891
  • Protein Accession#:
  • NP_114172;P14635
  • Gene Name:
  • CCNB1
  • Gene Alias:
  • CCNB
  • Gene Description:
  • cyclin B1
  • Gene Summary:
  • The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq
  • Other Designations:
  • G2/mitotic-specific cyclin B1
  • Interactome
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